Spermine synthase Recombinant Rabbit Monoclonal Antibody [JE61-85]
Catalog# HA720065
Spermine synthase Recombinant Rabbit Monoclonal Antibody [JE61-85]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
予測される交差反応性
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unconjugated
製品概要
製品名
Spermine synthase Recombinant Rabbit Monoclonal Antibody [JE61-85]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Spermine synthase aa 100-150/366.
交差反応性（対応種属）
Human, Mouse (Predicted: Rat)
予測される交差反応性
検証済みアプリケーション
WB, IF-Cell, IHC-P
分子量
41 kDa
ポジティブコントロール
Hela cell lysate, mouse testis tissue lysate, human placenta tissue, human prostate tissue, Hela.
コンジュゲーション
unconjugated
クローン番号
JE61-85
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
WB
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1:500-1:2,000
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IF-Cell
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1:50
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IHC-P
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1:50-1:200
ターゲット
機能
This gene encodes a protein belonging to the spermidine/spermin synthase family and catalyzes the production of spermine from spermidine. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene cause an X-linked intellectual disability called Snyder-Robinson Syndrome (SRS). Multiple transcript variants encoding different isoforms have been found for this gene. Spermine synthase is an enzyme that converts spermidine into spermine. This enzyme catalyses the following chemical reaction. Spermine synthase is a form of polyamine that is in all eukaryotes and plays a role in a variety of biological functions in plants. Its structure consists of two identical monomers of 41 kDa with three domains each, creating a homodimer formed via dimerization. The interactions between one of the three domains, the N-terminals of the monomers, is responsible for dimerization as that is where the active site is located; the central terminal consisting of four β- strands structurally forming a lid for the third domain, the C-terminal domain.
背景・参考文献
1. Guo Y. et. al. Spermine synthase and MYC cooperate to maintain colorectal cancer cell survival by repressing Bim expression. Nat Commun. 2020 Jun
2. Timson DJ. Myosin Va and spermine synthase: partners in exosome transport. Biosci Rep. 2019 Apr
サブセルラー局在
Cytosol, extracellular exosome.
別名
MRSR antibody
SMS antibody
Snyder Robinson X linked mental retardation syndrome antibody
Spermidine aminopropyltransferase antibody
Spermine synthase antibody
SPMSY antibody
SpS antibody
SPSY_HUMAN antibody
SRS antibody
画像
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Western blot analysis of Spermine synthase on different lysates with Rabbit anti-Spermine synthase antibody (HA720065) at 1/1,000 dilution.
Lane 1: HeLa
Lane 2: Mouse testis
Lysates/proteins at 10 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA720065, 1/1,000 in primary antibody dilution buffer (K1803), room temperature for 2 hours.
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 41.3 kDa
Observed band size: 41 kDa -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Spermine synthase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720065, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Spermine synthase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720065, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunocytochemistry analysis of Hela cells labeling Spermine synthase with Rabbit anti-Spermine synthase antibody (HA720065) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Spermine synthase antibody (HA720065) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）