c-Fos Recombinant Antibody [PSH07-51] - Mouse IgG1 (Chimeric)
Catalog# HA601440
c-Fos Recombinant Antibody [PSH07-51] - Mouse IgG1 (Chimeric)
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IHC-Fr
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IHC-P
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IF-Cell
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WB
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
予測される種属に対するアフターサービスのサポート
概要
製品名
c-Fos Recombinant Antibody [PSH07-51] - Mouse IgG1 (Chimeric)
抗体の種類
Recombinant Chimeric Antibody
免疫原
Recombinant protein within human Protein c-Fos aa 1-380.
種属反応性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される種属に対するアフターサービスのサポート
検証された応用例
IHC-Fr, IHC-P, IF-Cell, WB
分子量
Predicted band size: 41 kDa
陽性対照
Mouse brain tissue, rat brain tissue.
結合
unconjugated
クローン番号
PSH07-51
製品の特徴
形態
Liquid
濃度
1ug/ul
保存方法
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
応用希釈度
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IHC-Fr
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1:1,000-1:5,000
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IHC-P
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1:5,000
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IF-Cell
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1:1,000
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WB
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1:5,000-1:10,000
ターゲット
機能
Protein c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos. It is encoded in humans by the FOS gene. It was first discovered in rat fibroblasts as the transforming gene of the FBJ MSV (Finkel–Biskis–Jinkins murine osteogenic sarcoma virus). It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2. It has been mapped to chromosome region 14q21→q31. c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression. It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.
背景文献
1. Matsuoka K et al. Metabolic rewiring controlled by c-Fos governs cartilage integrity in osteoarthritis. Ann Rheum Dis. 2023 Sep
2. Osada N et al. c-FOS is an integral component of the IKZF1 transactivator complex and mediates lenalidomide resistance in multiple myeloma. Clin Transl Med. 2023 Aug
亜細胞局在
Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.
別名
Activator protein 1 antibody
AP 1 antibody
C FOS antibody
Cellular oncogene c fos antibody
Cellular oncogene fos antibody
FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
FBJ murine osteosarcoma viral oncogene homolog antibody
FBJ murine osteosarcoma viral v fos oncogene homolog antibody
FBJ Osteosarcoma Virus antibody
FOS antibody
展開画像
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-c-Fos antibody (HA601440) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601440) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-c-Fos antibody (HA601440) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601440) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa cells untreated / HeLa cells starved 16 hours then treated with 200nM TPA for 4 hours labeling c-Fos with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells untreated / C6 cells starved 16 hours then treated with 200nM TPA for 4 hours labeling c-Fos with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Western blot analysis of c-Fos on different lysates with Mouse anti-c-Fos antibody (HA601440) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41-55 kDa
Exposure time: 59 seconds; ECL: K1801;
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601440) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"