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GPX4 Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER1803-15

GPX4 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

GPX4 Rabbit Polyclonal Antibody

抗体の種類

Rabbit Polyclonal Antibody

免疫原

Recombinant protein within Human GPX4 aa 1-100 / 197.

種属反応性

Human, Mouse, Rat

検証された応用例

WB, IF-Cell, IHC-P, FC

分子量

Predicted band size: 22 kDa

陽性対照

Rat testis tissue lysate, HepG2, LOVO, MCF-7, mouse testis tissue.

結合

unconjugated

RRID

AB_3069214

製品の特徴

形態

Liquid

濃度

1ug/ul

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Immunogen affinity purified.

応用希釈度

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:50 -1:600

  • FC

  • 1:50-1:100

ターゲット

機能

Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins . Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide . Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species . The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis . The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures . May be required to protect cells from the toxicity of ingested lipid hydroperoxides . Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion.

背景文献

1. Yang W S et al. Regulation of ferroptotic cancer cell death by GPX4. Cell 156:317-331(2014).

2. Scheerer P. Structural basis for catalytic activity and enzyme polymerization of phospholipid hydroperoxide glutathione peroxidase-4 (GPx4). Biochemistry 46:9041-9049(2007).

配列相同性

Belongs to the glutathione peroxidase family.

組織特異性

Present primarily in testis. Expressed in platelets (at protein level).

亜細胞局在

Cytoplasm. Mitochondrion.

UNIPROT #

SwissProt: P36969 Human

SwissProt: O70325 Mouse

SwissProt: P36970 Rat

別名

Glutathione peroxidase 4 antibody

GPX 4 antibody

GPX-4 antibody

GPX4 antibody

GPX4_HUMAN antibody

GSHPx-4 antibody

MCSP antibody

mitochondrial antibody

PHGPx antibody

Phospholipid hydroperoxidase antibody

展開

画像

  • Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (<a href="/products/ER1803-15" style="font-weight: bold;text-decoration: underline;">ER1803-15</a>) at 1/1,000 dilution.<br /><br />Lane 1: Rat testis tissue lysate (20 µg/Lane)<br />Lane 2: Mouse testis tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 2 minutes;<br /><br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1803-15" style="font-weight: bold;text-decoration: underline;">ER1803-15</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ER1803-15) at 1/1,000 dilution.

    Lane 1: Rat testis tissue lysate (20 µg/Lane)
    Lane 2: Mouse testis tissue lysate (20 µg/Lane)

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 2 minutes;

    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-15) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin.

  • Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

Filter:
  1. WB
  2. IP
  3. IF

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