GPX4 Rabbit Polyclonal Antibody
Catalog# ER1803-15
GPX4 Rabbit Polyclonal Antibody
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WB
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Mouse
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Rat
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unconjugated
製品概要
製品名
GPX4 Rabbit Polyclonal Antibody
抗体のタイプ
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within Human GPX4 aa 1-100 / 197.
交差反応性（対応種属）
Mouse, Rat
検証済みアプリケーション
WB
分子量
Predicted band size: 22 kDa
ポジティブコントロール
Rat testis tissue lysate, mouse testis tissue lysate.
コンジュゲーション
unconjugated
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Immunogen affinity purified.
推奨希釈倍率
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WB
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1:1,000
ターゲット
機能
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins . Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide . Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species . The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis . The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures . May be required to protect cells from the toxicity of ingested lipid hydroperoxides . Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion.
背景・参考文献
1. Yang W S et al. Regulation of ferroptotic cancer cell death by GPX4. Cell 156:317-331(2014).
2. Scheerer P. Structural basis for catalytic activity and enzyme polymerization of phospholipid hydroperoxide glutathione peroxidase-4 (GPx4). Biochemistry 46:9041-9049(2007).
配列相同性
Belongs to the glutathione peroxidase family.
組織特異性
Present primarily in testis. Expressed in platelets (at protein level).
サブセルラー局在
Cytoplasm. Mitochondrion.
別名
Glutathione peroxidase 4 antibody
GPX 4 antibody
GPX-4 antibody
GPX4 antibody
GPX4_HUMAN antibody
GSHPx-4 antibody
MCSP antibody
mitochondrial antibody
PHGPx antibody
Phospholipid hydroperoxidase antibody
詳細を見る画像
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Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ER1803-15) at 1/1,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1minutes 2seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1803-15, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22.2 kDa
Observed band size: 22.2 kDa
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
文献・引用論文
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Identification of Pinostilbene as a natural STING agonist that triggers FTH1 degradation via K48-ubiquitination to induce ferroptosis in non-small cell lung cancer
ジャーナル: Redox Biology
DOI: 10.1016/j.redox.2026.104099
IF: 11.9
アプリケーション: WB
交差反応性: Human
掲載日: 2026 Feb
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Z-Guggulsterone Induces Ferroptosis in Chemoresistant Breast Cancer via a CDKN2B-Mediated Suppression of GTP Cyclohydrolase 1
ジャーナル: Phytotherapy Research
DOI: 10.1002/ptr.70326
IF: 6.3
アプリケーション: WB
交差反応性: Human
掲載日: 2026 Apr
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Zinc ions attenuates iridovirus infection through regulation of ferroptosis pathways
ジャーナル: Cell Death Discovery
DOI: 10.1038/s41420-026-03114-x
IF: 7
アプリケーション: WB
交差反応性: Fish
掲載日: 2026 Apr
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Computational-Experimental Integration Reveals PPARγ/Nrf2-GPX4 Dual-Targeting by Combined Selenomethionine-Magnolol as a Novel Therapy for Sarcopenic Obesity
ジャーナル: Journal of Assisted Reproduction and Genetics
DOI: 10.1016/j.freeradbiomed.2026.04.013
IF: 8.2
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 Apr
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UCHL1 enhances TSC1 transcription by stabilizing FOXO1 through deubiquitination in knee osteoarthritis
ジャーナル: Histology and Histopathology
DOI: 10.14670/HH-18-995
IF: 2
アプリケーション: WB
交差反応性: Human,Rat
掲載日: 2025 Sept
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Deficiency of Vitamin D-binding protein exacerbates liver fibrosis by disrupting iron homeostasis via the activation of YAP signaling
ジャーナル: Experimental Cell Research
DOI: 10.1016/j.yexcr.2025.114767
IF: 3.5
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Sept
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Senkyunolide I Alleviated Renal Fibrosis in UUO Mice by Regulating the Nrf2/xCT/GPX4 and NLRP3/Caspase-1/GSDMD Pathways to Inhibit Ferroptosis and Pyroptosis
ジャーナル: FASEB Journal
DOI: 10.1096/fj.202502377R
IF: 4.2
アプリケーション: WB
交差反応性: Mouse,Rat
掲載日: 2025 Oct
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WGX50 attenuates radiation enteritis by targeting ferroptosis and redox homeostasis via EGFR
ジャーナル: Molecular Medicine
DOI: 10.1186/s10020-025-01375-3
IF: 6.4
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Oct
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Trimetazidine alleviates heart failure after myocardial infarction by promoting PINK1/parkin-mediated mitophagy and suppressing GPX4-dependent ferroptosis
ジャーナル: European Journal Of Pharmacology
DOI: 10.1016/j.ejphar.2025.178376
IF: 4.7
アプリケーション: IHC,WB
交差反応性: Mouse
掲載日: 2025 Nov
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AATF alleviates cerebral ischemia-reperfusion injury by suppressing endoplasmic reticulum stress-induced ferroptosis through upregulating the PI3K/Akt pathway
ジャーナル: Neurological Research
DOI: 10.1080/01616412.2025.2564226
IF: 1.5
アプリケーション: WB
交差反応性: Rat
掲載日: 2025 Nov
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Design of photoactivatable methylene blue-bufalin conjugate for GPX4-targeted degradation to induce ferroptosis-like death in breast cancer therapy
ジャーナル: Bioorganic Chemistry
DOI: 10.1016/j.bioorg.2025.108629
IF: 4.5
アプリケーション: WB
交差反応性: Human
掲載日: 2025 May
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Vitamin C Inhibits Scale Drop Disease Virus Infectivity by Targeting Nrf2 to Reduce Ferroptosis
ジャーナル: Antioxidants
DOI: 10.3390/antiox14050576
IF: 6
アプリケーション: WB
交差反応性: Fish
掲載日: 2025 May
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Exploring the Mechanisms of the Ferroptosis-Related Gene TGFBR1 in Autoimmune Uveitis Based on Machine Learning Models
ジャーナル: ACS Omega
DOI: 10.1021/acsomega.5c00595
IF: 4.3
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Jun
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5‐HT7R Deficiency Alleviates ADP‐Heptose‐Induced Cognitive Impairment via Inhibiting Ferroptosis and Neuroinflammation in Mice
ジャーナル: CNS Neuroscience & Therapeutics
DOI: 10.1111/cns.70455
IF: 5
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Jun
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Linarin alleviates high-fat diet-induced hepatic steatosis by inhibiting PDE4D and activating the cAMP/PKA/CREB pathway.
ジャーナル: Free Radical Biology And Medicine
DOI: 10.1016/j.freeradbiomed.2025.07.030
IF: 8.2
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Jul
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3-hydroxyanthranilic acid analog mitigates ischemia-reperfusion injury by inhibiting cardiomyocyte ferroptosis through the activation of Nrf2/HO-1/GPX4
ジャーナル: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.115117
IF: 4.7
アプリケーション: WB
交差反応性: Rat
掲載日: 2025 Jul
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Programmable Biporphyrin-G-Quadruplex Nanoflowers for Simultaneous Tumor Cell Recognition and Enhanced Photodynamic Therapy
ジャーナル: Small Methods
DOI: 10.1002/smtd.202401993
IF: 9.1
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Jul
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A lactylation-ferroptosis cross-talk gene signature predicts hepatocellular carcinoma prognosis and reveals STMN1/PRDX1 as therapeutic targets
ジャーナル: Frontiers In Immunology
DOI: 10.3389/fimmu.2025.1677089
IF: 5.9
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Dec
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Vitamin D alleviates diabetic kidney damage via TREM2-dependent modulation of ferritinophagy and macrophage polarization
ジャーナル: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.116076
IF: 4.7
アプリケーション: WB
交差反応性: Rat
掲載日: 2025 Dec
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The Chemotherapy Medication of Evodia lepta (Spreng). Merr. on the Viability of Tongue Cancer Cells Through the PD-L1/MMP14/HSPA5 Pathway
ジャーナル: Cancer Management And Research
DOI: 10.2147/CMAR.S533380
IF: 2.6
アプリケーション: WB
交差反応性: Human,Mouse
掲載日: 2025 Aug
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LncRNA OIP5-AS1 regulates ferroptosis and mitochondrial dysfunction-mediated apoptosis in spinal cord injury by targeting the miR-128-3p/Nrf2 axis
ジャーナル: Heliyon
DOI: 10.1016/j.heliyon.2024.e37704
IF: 3.4
アプリケーション: WB
交差反応性: Mouse
掲載日: 2024 Sept
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Corydalis yanhusuo extract and its pharmacological substances alleviate food allergy by inhibiting mast cells activation via PLC/PKC/STAT3 pathway
ジャーナル: Journal Of Ethnopharmacology
DOI:
IF: 6.1
アプリケーション: WB
交差反応性: Rat
掲載日: 2024 Sep
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Biosynthetic deficiency of docosahexaenoic acid causes nonalcoholic fatty liver disease and ferroptosis-mediated hepatocyte injury
ジャーナル: Journal Of Biological Chemistry
DOI:
IF: 4.8
アプリケーション: WB
交差反応性: Zebrafish
掲載日: 2024 May
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Heme oxygenase-1 is involved in the erastin-induced ferroptosis in acute myeloid leukemia cells
ジャーナル: Preprint And Has Not Been Certified By Peer Review
DOI:
IF:
アプリケーション: WB
交差反応性: Human
掲載日: 2024 May
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A DNA tetrahedron-based ferroptosis-suppressing nanoparticle: superior delivery of curcumin and alleviation of diabetic osteoporosis
ジャーナル: Bone Research
DOI:
IF: 12.7
アプリケーション: IF-cell
交差反応性: Mouse
掲載日: 2024 Mar
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Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling
ジャーナル: Cellular Signalling
DOI:
IF: 4.8
アプリケーション: WB
交差反応性: Mouse
掲載日: 2024 Feb
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CAPE activates AMPK and Foxo3 signaling to induce growth inhibition and ferroptosis in triple-negative breast cancer
ジャーナル: Public Library Of Science One
DOI:
IF: 2.9
アプリケーション: WB
交差反応性: Human
掲載日: 2024 Dec
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Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis
ジャーナル: Redox Biology
DOI:
IF: 11.4
アプリケーション: WB
交差反応性: Mouse
掲載日: 2024 Apr
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Carnosine attenuates renal ischemia-reperfusion injury by inhibiting GPX4-mediated ferroptosis
ジャーナル: International Immunopharmacology
DOI:
IF: 5.6
アプリケーション: WB
交差反応性: Mouse
掲載日: 2023 Nov
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Anomanolide C suppresses tumor progression and metastasis by ubiquitinating GPX4-driven autophagy-dependent ferroptosis in triple negative breast cancer
ジャーナル: International Journal Of Biological Sciences
DOI:
IF: 10.750
アプリケーション: WB,IP
交差反応性: Mouse
掲載日: 2023 May
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Secondary iron overload induces chronic pancreatitis and ferroptosis of acinar cells in mice
ジャーナル: International Journal Of Molecular Medicine
DOI:
IF: 5.314
アプリケーション: WB
交差反応性: Mouse
掲載日: 2023 Jan
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H1N1 influenza virus infection through NRF2-KEAP1-GCLC pathway induces ferroptosis in nasal mucosal epithelial cells
ジャーナル: Free Radical Biology And Medicine
DOI:
IF: 7.4
アプリケーション: WB
交差反応性: Mouse
掲載日: 2023 Aug
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High Hepcidin Levels Promote Abnormal Iron Metabolism and Ferroptosis in Chronic Atrophic Gastritis
ジャーナル: Biomedicines
DOI: 10.3390/biomedicines11092338
IF: 4.7
アプリケーション: WB
交差反応性: Rat
掲載日: 2023 Aug
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Artemisitene Alters LPS-Induced Oxidative stress, inflammation and Ferroptosis in Liver Through Nrf2/HO-1 and NF-kB Pathway
ジャーナル: Frontiers In Pharmacology
DOI:
IF: 5.6
アプリケーション: WB
交差反応性: Mouse
掲載日: 2023 Apr
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Hydroxysafflor yellow A and anhydrosafflor yellow B alleviate ferroptosis and parthanatos in PC12ácells injured by OGD/R
ジャーナル: Free Radical Biology And Medicine
DOI:
IF: 7.4
アプリケーション: WB
交差反応性: Rat
掲載日: 2022 Feb
Alternative Products
GPX4 Recombinant Rabbit Monoclonal Antibody [JU11-31]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat,Zebrafish,Pig,Cynomolgus monkey
Conjugate: unconjugated
同一ターゲット＆同一シグナル伝達経路の関連製品
GPX4 Recombinant Rabbit Monoclonal Antibody [JU11-31]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat,Zebrafish,Pig,Cynomolgus monkey
Conjugate: unconjugated
