Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]
Catalog# ET1702-59
Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
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HA751846
不含抗保成分
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unconjugated
製品概要
製品名
Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human GLP1 aa 65-150.
製品の特異性
Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10] recognizes endogenous levels of GLP-1 and total glucagon protein.
交差反応性（対応種属）
Human, Mouse, Rat
検証済みアプリケーション
WB, IF-Tissue, IHC-P
標的分子量
Predicted band size: 21 kDa
ポジティブコントロール
human pancreas tissue.
コンジュゲーション
unconjugated
クローン番号
JF33-10
RRID
Reactivity Data
検証済（社内検証通過）
文献報告済（社内未検証、文献サポートあり）
反応性予測（高い相同性に基づく）
非推奨（社内検証未通過）
| IHC-P | IF-Tissue | |
|---|---|---|
| human |
|
|
| mouse |
|
|
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
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WB
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1:500-1:1,000
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IF-Tissue
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1:100
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IHC-P
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1:1,000
ターゲット
機能
Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the Glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone.
背景・参考文献
1. Takada Y et al. Brg1 plays an essential role in development and homeostasis of the duodenum through regulation of Notch signaling. Development 143:3532-3539 (2016).
2. Jiang S et al. AMPK-dependent regulation of GLP1 expression in L-like cells. J Mol Endocrinol 57:151-60 (2016).
配列相同性
Belongs to the glucagon family.
組織特異性
Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
翻訳後修飾（PTM）
Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
サブセルラー局在
Secreted.
別名
GCG antibody
glicentin-related polypeptide antibody
GLP-1 antibody
GLP-1(7-36) antibody
GLP-1(7-37) antibody
GLP-2 antibody
GLP1 antibody
GLP1, included antibody
GLP2 antibody
GLP2, included antibody
詳細を見る画像
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Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Glucagon with Rabbit anti-Glucagon antibody (ET1702-59) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-59, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Glucagon antibody (ET1702-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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