GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]
Catalog# HA721338
GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]
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WB
-
IHC-P
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IF-Tissue
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Human
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Mouse
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unconjugated
製品概要
製品名
GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human GABA B Receptor 1 aa 35-134 / 961.
交差反応性（対応種属）
Human, Mouse
検証済みアプリケーション
WB, IHC-P, IF-Tissue
標的分子量
Predicted band size: 108 kDa
ポジティブコントロール
K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, SHG-44 cell lysate, mouse brain tissue.
コンジュゲーション
unconjugated
クローン番号
JE60-09
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:100
ターゲット
機能
Gamma-aminobutyric acid B receptor, 1 (GABAB1), is a G-protein coupled receptor subunit encoded by the GABBR1 gene. GABAB1 is a receptor for Gamma-aminobutyric acid. Upon binding, GABAB1 will produce a slow and prolonged inhibitory effect. GABAB1 is one part of a heterodimer, which is the GABAB receptor, consisting of it and the related GABAB2 protein. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. GABBR1 has been shown to interact with ATF4 and GABBR2.
背景・参考文献
1. Shao L et al. The neurotransmitter receptor Gabbr1 regulates proliferation and function of hematopoietic stem and progenitor cells. Blood. 2021 Feb
2. Cediel ML et al. GABBR1 monoallelic de novo variants linked to neurodevelopmental delay and epilepsy. Am J Hum Genet. 2022 Oct
サブセルラー局在
Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted.
別名
dJ271M21.1.1 antibody
dJ271M21.1.2 antibody
FLJ92613 antibody
GABA-B receptor 1 antibody
GABA-B-R1 antibody
GABA-BR1 antibody
GABAB R1 antibody
GABAB subunit 1c antibody
GABABR1 antibody
GABBR1 3 antibody
詳細を見る画像
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721338) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721338, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 4: SHG-44 (Human glioma cell) cell lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 5 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721338, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 108 kDa
Observed band size: 108 kDa
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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