Skip to content

COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04]

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# ET1609-68

COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Predicted reactivity

  • Cow

予測される交差反応性
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04]

抗体のタイプ

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human COL1A1 aa 1191-1238 / 1464.

交差反応性（対応種属）

Human (Predicted: Cow)

予測される交差反応性

検証済みアプリケーション

WB, IHC-P

分子量

Predicted band size: 139 kDa

ポジティブコントロール

human kidney tissue, human colon carcinoma tissue.

コンジュゲーション

unconjugated

クローン番号

ST58-04

RRID

AB_3069877

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

推奨希釈倍率

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

ターゲット

機能

Collagen, type I, alpha 1, also known as alpha-1 type I collagen, is a protein that in humans is encoded by the COL1A1 gene. COL1A1 encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. Collagen is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera). The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain. This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong mature type I collagen fibers. Collagenous function includes rigidity and elasticity.

背景・参考文献

1. Dang PN et al. Controlled Dual Growth Factor Delivery From Microparticles Incorporated Within Human Bone Marrow-Derived Mesenchymal Stem Cell Aggregates for Enhanced Bone Tissue Engineering via Endochondral Ossification. Stem Cells Transl Med 5:206-17 (2016).

2. Huang W et al. Astragalus and Paeoniae radix rubra extract inhibits liver fibrosis by modulating the transforming growth factor- /Smad pathway in rats. Mol Med Rep 11:805-14 (2015).

配列相同性

Belongs to the fibrillar collagen family.

組織特異性

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

翻訳後修飾（PTM）

Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.; Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.; Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.; O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

サブセルラー局在

Secreted.

UNIPROT #

SwissProt: P02452 Human

Entrez Gene:282187 Cow

別名

Alpha 1 type I collagen antibody

alpha1(I) procollagen antibody

CO1A1_HUMAN antibody

COL1A1 antibody

collagen alpha 1 chain type I antibody

Collagen alpha-1(I) chain antibody

collagen alpha-1(I) chain preproprotein antibody

Collagen I alpha 1 polypeptide antibody

collagen of skin, tendon and bone, alpha-1 chain antibody

Collagen type I alpha 1 antibody

詳細を見る

画像

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COL1A1 antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COL1A1 antibody (ET1609-68) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-COL1A1 antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-COL1A1 antibody (ET1609-68) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of COL1A1 on HFF-1 cell lysate with Rabbit anti-COL1A1 antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 59 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 139 kDa<br />Observed band size: 250 kDa

    Western blot analysis of COL1A1 on HFF-1 cell lysate with Rabbit anti-COL1A1 antibody (ET1609-68) at 1/5,000 dilution.

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1609-68, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 139 kDa
    Observed band size: 250 kDa

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

文献・引用論文

同一ターゲット＆同一シグナル伝達経路の関連製品

metafield image

COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04] - BSA and Azide free

Application: WB,IHC-P

Reactivity: Human,Cow

Conjugate: unconjugated

metafield image

COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH06-20] - BSA and Azide free

Application: WB,IHC-P,IF-Tissue,IF-Cell,FC,IP,IHC-Fr

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

COL1A1 Recombinant Rabbit Monoclonal Antibody

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

metafield image

COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH06-20]

Application: WB,IHC-P,IF-Tissue,IF-Cell,FC,IP,mIHC,IHC-Fr

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单