ERK1/2 Rabbit Polyclonal Antibody
Catalog# ER131011
ERK1/2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
製品概要
製品名
ERK1/2 Rabbit Polyclonal Antibody
抗体のタイプ
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human ERK1 aa 306-360.
交差反応性（対応種属）
Human, Mouse, Rat
検証済みアプリケーション
WB, IF-Cell, IHC-P
分子量
Predicted band size: 43/41 kDa
ポジティブコントロール
HeLa cell lysate, Jurkat cell lysate, Ramos cell lysate, MCF7 cell lysate, Neuro-2a cell lysate, C6 cell lysate, HeLa, RAW264.7, PC-12, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue.
コンジュゲーション
unconjugated
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Immunogen affinity purified.
推奨希釈倍率
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WB
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1:2,000-1:5,000
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IF-Cell
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1:200
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IHC-P
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1:200
ターゲット
機能
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.
背景・参考文献
1. "Tumor suppressor density-enhanced phosphatase-1 (DEP-1) inhibits the RAS pathway by direct dephosphorylation of ERK1/2 kinases." Sacco F., Tinti M., Palma A., Ferrari E., Nardozza A.P., Hooft van Huijsduijnen R., Takahashi T., Castagnoli L., Cesareni G. J. Biol. Chem. 284:22048-22058(2009)
2. "A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy." Lorenz K., Schmitt J.P., Schmitteckert E.M., Lohse M.J. Nat. Med. 15:75-83(2009)
3. "The ERK cascade: distinct functions within various subcellular organelles." Wortzel I., Seger R. Genes Cancer 2:195-209(2011)
配列相同性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
翻訳後修飾（PTM）
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
サブセルラー局在
Cytoplasm, Nucleus, Cell junction, Membrane.
UNIPROT #
別名
ERK 1 antibody
ERK 2 antibody
ERK-2 antibody
ERK1 antibody
erk1/2 antibody
ERK2 antibody
ERT1 antibody
ERT2 antibody
Extracellular signal regulated kinase 1 antibody
Extracellular signal-regulated kinase 2 antibody
詳細を見る画像
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Western blot analysis of ERK1/2 on different lysates with Rabbit anti-ERK1/2 antibody (ER131011) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Ramos cell lysate
Lane 4: MCF7 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43/41 kDa
Observed band size: 43/41 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER131011) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling ERK1/2 with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of RAW264.7 cells labeling ERK1/2 with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling ERK1/2 with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1/2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK1/2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-ERK1/2 antibody. Counter stained with hematoxylin.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
文献・引用論文
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HBx Induces the Production of N-GPC3 in Hepatoma Cells to Promote the Evasion of Macrophage Phagocytosis Through Activating Furin
ジャーナル: Journal Of Cellular And Molecular Medicine
DOI: 10.1111/jcmm.71122
IF: 4.2
アプリケーション: WB
交差反応性: Human
掲載日: 2026 Apr
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A dihydrofuro[2,3-b] benzofuran derivative alleviates lipopolysaccharide induced acute lung injury via suppressing MAPK signaling
ジャーナル: Frontiers In Pharmacology
DOI: 10.3389/fphar.2026.1763318
IF: 4.8
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 Apr
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Peptidomimetic Long-Acting Growth Hormone-Releasing Hormone Agonists Promote Tissue Perfusion in Hindlimb Ischemia
ジャーナル: Journal Of Medicinal Chemistry
DOI: 10.1021/acs.jmedchem.5c03408
IF: 6.8
アプリケーション: WB
交差反応性: Human
掲載日: 2026 Apr
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Collagen peptides derived from sea bass scales (Lateolabrax maculatus) relieve photoaging through MAPK, NF-κB and TGF-β signaling pathways and modulating gut microbiota composition
ジャーナル: Food Research International
DOI: 10.1016/j.foodres.2025.117521
IF: 8
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Sept
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In vitro and clinical evaluation of platelet-rich plasma combined with angioplasty in diabetic foot treatment
ジャーナル: World Journal of Diabetes
DOI: 10.4239/wjd.v16.i10.110631
IF: 4.6
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Oct
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A Sprayable Temperature-Sensitive Hydrogel Promotes Oral Ulcer Healing through NGFR/ERK Signaling Pathway
ジャーナル: ACS Applied Materials & Interfaces
DOI: 10.1021/acsami.5c02326
IF: 8.3
アプリケーション: WB
交差反応性: Human
掲載日: 2025 May
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The Piezo1/Extracellular Signal-Regulated Kinase Signal Pathway Regulates Proliferation and Migration of Aortic Vascular Smooth Muscle Cells and Participates in Thoracic Aortic Aneurysm
ジャーナル: Heart Lung and Circulation
DOI: 10.1016/j.hlc.2025.03.012
IF: 2.2
アプリケーション: WB
交差反応性: Human,Rat
掲載日: 2025 Jun
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Dihydrotanshinone I enhanced BRAF mutant melanoma treatment efficacy by inhibiting the STAT3/SOX2 signaling pathway
ジャーナル: Frontiers in Oncology
DOI: 10.3389/fonc.2025.1429018
IF: 3.3
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Jan
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USP44 Regulates Chemoresistance Induced by ROS and the MAPK/NF-κB Pathway Through the Stabilization of ITGB4 in Gastric Cancer
ジャーナル: FASEB Journal
DOI: 10.1096/fj.202501369R
IF: 4.2
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Aug
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