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COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04]

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Catalog# ET1609-68

COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Predicted reactivity

  • Cow

予測される種属に対するアフターサービスのサポート
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04]

抗体の種類

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human COL1A1 aa 1191-1238 / 1464.

種属反応性

Human (Predicted: Cow)

予測される種属に対するアフターサービスのサポート

検証された応用例

WB, IHC-P

分子量

Predicted band size: 139 kDa

陽性対照

Human placenta tissue lysates, human skin tissue lysates, human kidney tissue, human colon carcinoma tissue.

結合

unconjugated

クローン番号

ST58-04

RRID

AB_3069877

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

応用希釈度

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:1,000

ターゲット

機能

Collagen, type I, alpha 1, also known as alpha-1 type I collagen, is a protein that in humans is encoded by the COL1A1 gene. COL1A1 encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. Collagen is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera). The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain. This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong mature type I collagen fibers. Collagenous function includes rigidity and elasticity.

背景文献

1. Dang PN et al. Controlled Dual Growth Factor Delivery From Microparticles Incorporated Within Human Bone Marrow-Derived Mesenchymal Stem Cell Aggregates for Enhanced Bone Tissue Engineering via Endochondral Ossification. Stem Cells Transl Med 5:206-17 (2016).

2. Huang W et al. Astragalus and Paeoniae radix rubra extract inhibits liver fibrosis by modulating the transforming growth factor- /Smad pathway in rats. Mol Med Rep 11:805-14 (2015).

配列相同性

Belongs to the fibrillar collagen family.

組織特異性

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

翻訳後修飾

Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.; Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.; Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.; O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

亜細胞局在

Secreted.

UNIPROT #

SwissProt: P02452 Human

Entrez Gene:282187 Cow

別名

Alpha 1 type I collagen antibody

alpha1(I) procollagen antibody

CO1A1_HUMAN antibody

COL1A1 antibody

collagen alpha 1 chain type I antibody

Collagen alpha-1(I) chain antibody

collagen alpha-1(I) chain preproprotein antibody

Collagen I alpha 1 polypeptide antibody

collagen of skin, tendon and bone, alpha-1 chain antibody

Collagen type I alpha 1 antibody

展開

画像

  • Western blot analysis of COL1A1 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of COL1A1 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of COL1A1 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of COL1A1 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COL1A1 antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COL1A1 antibody (ET1609-68) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-COL1A1 antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-68" style="font-weight: bold;text-decoration: underline;">ET1609-68</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-COL1A1 antibody (ET1609-68) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

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