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CD34 Recombinant Rabbit Monoclonal Antibody [PSH27-02] - BSA and Azide free

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Catalog# HA752059

CD34 Recombinant Rabbit Monoclonal Antibody [PSH27-02] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-Fr

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

製品概要

製品名

CD34 Recombinant Rabbit Monoclonal Antibody [PSH27-02] - BSA and Azide free

抗体のタイプ

Recombinant Rabbit monoclonal Antibody

交差反応性（対応種属）

Human, Mouse, Rat

検証済みアプリケーション

WB, IF-Cell, IHC-Fr, IHC-P

標的分子量

Predicted band size: 41 kDa

ポジティブコントロール

TF-1 (Human erythroleukemia cell) cell lysate.

コンジュゲーション

unconjugated

クローン番号

PSH27-02

Reactivity Data

Tested Tested Tested
検証済（社内検証通過）
Published Tested
文献報告済（社内未検証、文献サポートあり）
Predicted Tested
反応性予測（高い相同性に基づく）
Not recommended Tested
非推奨（社内検証未通過）
WB IHC-P IHC-Fr FC(Intra) IF-Cell
human
Tested Tested
Tested Tested
 
Not Recommended
Tested Tested
mouse
Not Recommended
Tested Tested
Tested Tested
 
Not Recommended
rat
Not Recommended
Tested Tested
 
 
 

製品の特徴

形態

Liquid

濃度

保存方法

Store at 2-8℃. Avoid freeze.

保存バッファー

PBS (pH7.4).

アイソタイプ

IgG

精製方法

Protein A affinity purified.

推奨希釈倍率

ターゲット

機能

CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. However, little is known about its exact function. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration.

背景・参考文献

1. Perret R et al. Superficial CD34-Positive Fibroblastic Tumor. Surg Pathol Clin. 2024 Mar

2. Rodrigues CR et al. CD34 Protein: Its expression and function in inflammation. Cell Tissue Res. 2023 Sep

サブセルラー局在

Membrane.

UNIPROT #

SwissProt: P28906 Human

SwissProt: Q64314 Mouse

Entrez Gene: 305081 Rat

別名

CD34 antibody

CD34 antigen antibody

CD34 molecule antibody

CD34_HUMAN antibody

Cluster designation 34 antibody

cluster of differentiation 34 antibody

Hematopoietic progenitor cell antigen CD34 antibody

HPCA1 antibody

Mucosialin antibody

OTTHUMP00000034733 antibody

詳細を見る

画像

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of CD34 on different lysates with Rabbit anti-CD34 antibody (<a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>) at 1/1,000 dilution. <br /> <br />Lane 1: TF-1 (Human erythroleukemia cell) cell lysate <br />Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1801 <br /> <br />Negative control: Daudi (PMID: 11606408). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 41 kDa <br />Observed band size: 80-120 kDa

    ☑ Relative expression (RE)

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Western blot analysis of CD34 on different lysates with Rabbit anti-CD34 antibody (HA724663) at 1/1,000 dilution.

    Lane 1: TF-1 (Human erythroleukemia cell) cell lysate
    Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Negative control: Daudi (PMID: 11606408).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724663, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 41 kDa
    Observed band size: 80-120 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: TF-1 (Human erythroleukemia cell) / Daudi (Human Burkitt's lymphoma cell) (negative) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). <br /> <br />Negative control: Daudi (PMID: 11606408).

    ☑ Relative expression (RE)

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: TF-1 (Human erythroleukemia cell) / Daudi (Human Burkitt's lymphoma cell) (negative)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724663, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Negative control: Daudi (PMID: 11606408).

  • This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Mouse <br />Tissue: Kidney <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br /> <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/250, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Kidney
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA724663, 1/250, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Cerebral cortex <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/2,500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Cerebral cortex
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/2,500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Cerebral cortex <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/2,500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Cerebral cortex
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/2,500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Cerebral cortex <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/2,500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Cerebral cortex
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724663" style="font-weight: bold;text-decoration: underline;">HA724663</a>, 1/2,500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724663, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724663, 1/2,500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

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