Skip to content

Vimentin Rabbit Polyclonal Antibody

製品仕様

今すぐ注文，【现货】

問い合わせをクリック

お問い合わせ

メール: [email protected]

電話: 0571-88062880

代理店を調べる

Catalog# R1308-6

Vimentin Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

予測される種属に対するアフターサービスのサポート
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

Vimentin Rabbit Polyclonal Antibody

抗体の種類

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within residues of Human Vimentin aa 417-466 / 466.

種属反応性

Human (Predicted: Mouse, Rat)

予測される種属に対するアフターサービスのサポート

検証された応用例

WB, IF-Cell, IHC-P

分子量

Predicted band size: 54 kDa

陽性対照

Hela cell lysate, A549 cell lysate, 293T cell lysate, Hela, human breast carcinoma tissue, Wild-type Hela whole cell lysate.

結合

unconjugated

RRID

AB_3073236

製品の特徴

形態

Liquid

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Immunogen affinity purified.

応用希釈度

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100-1:200

  • IHC-P

  • 1:200

ターゲット

機能

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two. Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways.

背景文献

1. "Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells." Perreau J., Lilienbaum A., Vasseur M., Paulin D. Gene 62:7-16(1988)

2. "Stabilization of vimentin coil2 fragment via an engineered disulfide." Chernyatina A.A., Strelkov S.V. J. Struct. Biol. 177:46-53(2012)

配列相同性

Belongs to the intermediate filament family.

組織特異性

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

翻訳後修飾

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

亜細胞局在

Cytoplasm, Cell membrane, Cytoskeleton, Intermediate filament, Membrane, Nucleus.

UNIPROT #

SwissProt: P08670 Human

別名

CTRCT30 antibody

Epididymis luminal protein 113 antibody

FLJ36605 antibody

HEL113 antibody

VIM antibody

VIME_HUMAN antibody

Vimentin antibody

画像

  • Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (<a href="/products/R1308-6" style="font-weight: bold;text-decoration: underline;">R1308-6</a>) at 1/2,000 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: 293T cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 1 minute;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1308-6" style="font-weight: bold;text-decoration: underline;">R1308-6</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (R1308-6) at 1/2,000 dilution.

    Lane 1: Hela cell lysate
    Lane 2: A549 cell lysate
    Lane 3: 293T cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 56 kDa

    Exposure time: 1 minute;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-6) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of Vimentin with anti-Vimentin antibody (<a href="/products/R1308-6" style="font-weight: bold;text-decoration: underline;">R1308-6</a>) at 1/500 dilution.<br />Lane 1: Wild-type Hela whole cell lysate (10 µg).<br />Lane 2: Vimentin knockout Hela whole cell lysate (10 µg).<br /><br /><a href="/products/R1308-6" style="font-weight: bold;text-decoration: underline;">R1308-6</a> was shown to specifically react with Vimentin in wild-type Hela cells. No band was observed when Vimentin knockout sample was tested. Wild-type and Vimentin knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1308-6" style="font-weight: bold;text-decoration: underline;">R1308-6</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of Vimentin with anti-Vimentin antibody (R1308-6) at 1/500 dilution.
    Lane 1: Wild-type Hela whole cell lysate (10 µg).
    Lane 2: Vimentin knockout Hela whole cell lysate (10 µg).

    R1308-6 was shown to specifically react with Vimentin in wild-type Hela cells. No band was observed when Vimentin knockout sample was tested. Wild-type and Vimentin knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1308-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescent staining of Hela cells using anti- vimentin rabbit polyclonal antibody.

    Immunofluorescent staining of Hela cells using anti- vimentin rabbit polyclonal antibody.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-vimentin rabbit polyclonal antibody.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-vimentin rabbit polyclonal antibody.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

Alternative Products

metafield image

Vimentin Recombinant Rabbit Monoclonal Antibody [PDH0-01]

Application: WB,IHC-P,IF-Cell,FC,IF-Tissue,mIHC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05]

Application: WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell,FC,IP

Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

同じターゲット & 同じ経路の製品

metafield image

Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05] - BSA and Azide free

Application: WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell,FC,IP

Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

metafield image

Vimentin Recombinant Rabbit Monoclonal Antibody [PDH0-01]

Application: WB,IHC-P,IF-Cell,FC,IF-Tissue,mIHC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

iFluor™ 647 Conjugated Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05]

Application: IF-Cell,IF-Tissue,FC

Reactivity: Human,Mouse,Rat

Conjugate: iFluor™ 647

metafield image

Vimentin Recombinant Rabbit Monoclonal Antibody

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

metafield image

Vimentin Mouse Monoclonal Antibody [D4-B11]

Application: WB,IF-Cell,IHC-P,FC,IF-Tissue,IHC-Fr,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Vimentin Recombinant Mouse Monoclonal Antibody [A6-C1-R] - BSA and Azide free

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05]

Application: WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell,FC,IP

Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

metafield image

Phospho-Vimentin (S39) Recombinant Rabbit Monoclonal Antibody [JE43-26]

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

iFluor™ 488 Conjugated Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05]

Application: IF-Cell,IF-Tissue,FC

Reactivity: Human,Mouse,Rat

Conjugate: iFluor™ 488

metafield image

Vimentin Recombinant Mouse Monoclonal Antibody [A6-C1-R]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Vimentin Mouse Monoclonal Antibody [A6-C1]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单