Tyrosine Hydroxylase Recombinant Rabbit Monoclonal Antibody [SD080-02] - BSA and Azide free
Catalog# HA750298
Tyrosine Hydroxylase Recombinant Rabbit Monoclonal Antibody [SD080-02] - BSA and Azide free
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WB
-
IHC-P
-
IF-Tissue
-
IHC-Fr
-
Human
-
Mouse
-
Rat
-
ET1612-65
含抗保成分
-
Cynomolgus monkey
予測される交差反応性
-
unconjugated
製品概要
製品名
Tyrosine Hydroxylase Recombinant Rabbit Monoclonal Antibody [SD080-02] - BSA and Azide free
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Tyrosine Hydroxylase aa 51-100 / 528.
交差反応性（対応種属）
Human, Mouse, Rat (Predicted: Cynomolgus monkey)
予測される交差反応性
検証済みアプリケーション
WB, IHC-P, IF-Tissue, IHC-Fr
標的分子量
Predicted band size: 59 kDa
ポジティブコントロール
PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue, mouse striatum tissue, rat striatum tissue.
コンジュゲーション
unconjugated
クローン番号
SD080-02
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
1*PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds.
背景・参考文献
1. Peng X et al. Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice. PLoS One 9:e90707 (2014).
2. Guo S et al. Optogenetic activation of the excitatory neurons expressing CaMKIIa in the ventral tegmental area upregulates the locomotor activity of free behaving rats. Biomed Res Int 2014:687469 (2014).
配列相同性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
組織特異性
Mainly expressed in the brain and adrenal glands.
サブセルラー局在
Cytoplasm.
別名
Dystonia 14 antibody
DYT14 antibody
DYT5b antibody
EC 1.14.16.2 antibody
OTTHUMP00000011225 antibody
OTTHUMP00000011226 antibody
ple antibody
Protein Pale antibody
TH antibody
The antibody
詳細を見る画像
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This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Striatum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: Striatum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Substantia nigra
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Striatum
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Western blot analysis of Tyrosine Hydroxylase on different lysates with Rabbit anti-Tyrosine Hydroxylase antibody (ET1612-65) at 1/5,000 dilution.
Lane 1: PC-12 cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-65) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tyrosine Hydroxylase antibody (ET1612-65) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-65) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tyrosine Hydroxylase antibody (ET1612-65) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-65) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Tyrosine Hydroxylase antibody (ET1612-65) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-65) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1612-65, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Substantia nigra
Sample: Paraffin-embedded section
Antibody concentration: 1:500
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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