Phospho-Beclin 1 (S30) Recombinant Rabbit Monoclonal Antibody [PSH16-40]
概要
製品名
Phospho-Beclin 1 (S30) Recombinant Rabbit Monoclonal Antibody [PSH16-40]
抗体の種類
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to residues surrounding Ser30 of human Beclin 1.
種属反応性
Human, Mouse, Rat
検証された応用例
WB
分子量
Predicted band size: 52 kDa
陽性対照
PC-12 cell lysate, PC-12 treated with 250nM Torin 1 for 2 hours cell lysate.
結合
unconjugated
クローン番号
PSH16-40
製品の特徴
形態
Liquid
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
応用希釈度
-
WB
-
1:10,000
ターゲット
機能
Beclin-1 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 is a mammalian ortholog of the yeast autophagy-related gene 6 (Atg6) and BEC-1 in the C. elegans nematode. This protein interacts with either BCL-2 or PI3k class III, playing a critical role in the regulation of both autophagy and cell death. Beclin-1 plays an important role in tumorigenesis, and neurodegeneration, being implicated in the autophagic programmed cell death. Ovarian cancer with upregulated autophagy has a less aggressive behavior and is more responsive to chemotherapy. Schizophrenia is associated with low levels of Beclin-1 in the hippocampus of those affected, which causes diminished autophagy which in turn results in increased neuronal cell death. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
背景文献
1. Hill SM et al. VCP/p97 regulates Beclin-1-dependent autophagy initiation. Nat Chem Biol. 2021 Apr
2. Liu Y et al. TLR9 and beclin 1 crosstalk regulates muscle AMPK activation in exercise. Nature. 2020 Feb
亜細胞局在
Cytoplasm, Golgi apparatus, trans-Golgi network membrane, Endosome membrane, Endoplasmic reticulum membrane, Mitochondrion membrane, Endosome, Cytoplasmic vesicle, autophagosome.
別名
APG6 antibody
ATG 6 antibody
ATG6 antibody
ATG6 autophagy related 6 homolog antibody
Bcl-2-interacting protein beclin antibody
Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
Beclin 1 autophagy related antibody
Beclin-1 antibody
Beclin1 antibody
BECN 1 antibody
展開画像
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-Beclin 1 (S30) on different lysates with Rabbit anti-Phospho-Beclin 1 (S30) antibody (HA723840) at 1/10,000 dilution.
Lane 1: PC-12 cell lysate
Lane 2: PC-12 treated with 250nM Torin 1 for 2 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 57 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723840) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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