Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24]
Catalog# HA722968
Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24]
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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HA751215
不含抗保成分
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Cynomolgus monkey
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Pig
予測される交差反応性
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unconjugated
製品概要
製品名
Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human OXT aa 1-125.
交差反応性（対応種属）
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される交差反応性
検証済みアプリケーション
WB, IHC-P, IHC-Fr, IF-Tissue
標的分子量
Predicted band size: 13 kDa
ポジティブコントロール
Mouse paraventricular nucleus tissue, rat supraoptic nucleus tissue, mouse pituitary tissue, rat pituitary tissue, Mouse pituitary tissue lysate, Rat pituitary tissue lysate.
コンジュゲーション
unconjugated
クローン番号
PSH08-24
Reactivity Data
| WB | IHC-P | IHC-Fr | IF-Tissue | |
|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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| Cynomolgus Monkey |
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| Pig |
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製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
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WB
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1:1,000
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IHC-P
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1:2,000-1:5,000
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IHC-Fr
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1:500-1:1,000
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IF-Tissue
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1:2,000
ターゲット
機能
Oxytocin is a peptide hormone and neuropeptide normally produced in the hypothalamus and released by the posterior pituitary. Present in animals since early stages of evolution, in humans it plays roles in behavior that include social bonding, love, reproduction, childbirth, and the period after childbirth. Oxytocin is released into the bloodstream as a hormone in response to sexual activity and during childbirth. It is also available in pharmaceutical form. In either form, oxytocin stimulates uterine contractions to speed up the process of childbirth. In its natural form, it also plays a role in maternal bonding and milk production. Production and secretion of oxytocin is controlled by a positive feedback mechanism, where its initial release stimulates production and release of further oxytocin. For example, when oxytocin is released during a contraction of the uterus at the start of childbirth, this stimulates production and release of more oxytocin and an increase in the intensity and frequency of contractions. This process compounds in intensity and frequency and continues until the triggering activity ceases. A similar process takes place during lactation and during sexual activity.
背景・参考文献
1. De Leon D et al. Methylation of OXT and OXTR genes, central oxytocin, and social behavior in female macaques. Horm Behav. 2020 Nov
2. Khazaal NM et al. The relationship between OXT gene polymorphisms and reproductive hormones in pregnant and lactating Awassi Ewes. Mol Biol Rep. 2023 Oct
サブセルラー局在
Secreted.
別名
MGC126890 antibody
MGC126892 antibody
Neurophysin 1 antibody
Neurophysin I antibody
Ocytocin antibody
OT antibody
OT NPI antibody
OXT antibody
Oxytocin antibody
Oxytocin Neurophysin preproprotein antibody
詳細を見る画像
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Application: IHC-Fr
Species: Mouse
Site: Paraventricular nucleus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Supraoptic nucleus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Pituitary
Sample: Paraffin-embedded section
Antibody concentration: 1/2,000 -
Application: IF-tissue
Species: Rat
Site: Pituitary
Sample: Paraffin-embedded section
Antibody concentration: 1/2,000 -
Immunohistochemical analysis of paraffin-embedded mouse paraventricular nucleus tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of Oxytocin-neurophysin 1 / OXT on different lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/1,000 dilution.
Lane 1: Mouse pituitary tissue lysate
Lane 2: Rat pituitary tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 13 kDa
Observed band size: 14 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722968) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
同一ターゲット＆同一シグナル伝達経路の関連製品
Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24] - BSA and Azide free
Application: WB,IHC-P,IHC-Fr,IF-Tissue
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
