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IL-6 Rabbit Polyclonal Antibody

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Catalog# R1412-2

IL-6 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

予測される交差反応性
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

IL-6 Rabbit Polyclonal Antibody

抗体のタイプ

Rabbit Polyclonal Antibody

免疫原

Recombinant protein within mouse IL6 aa 1-211.

交差反応性（対応種属）

Human (Predicted: Mouse, Rat)

予測される交差反応性

検証済みアプリケーション

WB, IF-Cell, IHC-P, FC

標的分子量

Predicted band size: 24 kDa

ポジティブコントロール

Recombinant murine Interleukin-6 protein, Siha, human spleen tissue.

コンジュゲーション

unconjugated

RRID

AB_3073311

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Immunogen affinity purified.

推奨希釈倍率

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50

ターゲット

機能

Cytokines play an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Interleukin-6 (IL-6) is a multifunctional cytokine with a wide variety of biological functions. IL-6 is implicated in the final differentiation of B-cells into immunoglobulin-secreting cells, myeloma and plasmacytoma growth, nerve cell differentiation, and activation of hepatocytes and mitogen-stimulated helper T cells.

背景・参考文献

1. "An IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in men infected with human immunodeficiency virus." Foster C.B., Lehrnbecher T., Samuels S., Stein S., Mol F., Metcalf J.A., Wyvill K., Steinberg S.M., Kovacs J., Blauvelt A., Yarchoan R., Chanock S.J.Blood 96:2562-2567(2000)

2. "A nucleotide variant in the promoter region of the interleukin-6 gene associated with decreased bone mineral density." Ota N., Nakajima T., Nakazawa I., Suzuki T., Hosoi T., Orimo H., Inoue S., Shirai Y., Emi M. J. Hum. Genet. 46:267-272(2001)

3. "Association of interleukin-6 promoter variant with bone mineral density in pre-menopausal women." Chung H.W., Seo J.-S., Hur S.E., Kim H.L., Kim J.Y., Jung J.H., Kim L.H., Park B.L., Shin H.D. J. Hum. Genet. 48:243-248(2003)

配列相同性

Belongs to the IL-6 superfamily.

翻訳後修飾（PTM）

N- and O-glycosylated.

サブセルラー局在

Secreted.

UNIPROT #

SwissProt: P05231 Human

SwissProt: P08505 Mouse

別名

Interleukin BSF 2 antibody

B cell differentiation factor antibody

B cell stimulatory factor 2 antibody

B-cell stimulatory factor 2 antibody

BSF 2 antibody

BSF-2 antibody

BSF2 antibody

CDF antibody

CTL differentiation factor antibody

Hepatocyte stimulatory factor antibody

詳細を見る

画像

  • ICC staining of IL-6 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/R1412-2" style="font-weight: bold;text-decoration: underline;">R1412-2</a>, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of IL-6 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1412-2, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1412-2" style="font-weight: bold;text-decoration: underline;">R1412-2</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1412-2, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of IL-6 was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/R1412-2" style="font-weight: bold;text-decoration: underline;">R1412-2</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of IL-6 was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (R1412-2, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Western blot analysis of IL-6 on different lysates with Rabbit anti-IL-6 antibody (<a href="/products/R1412-2" style="font-weight: bold;text-decoration: underline;">R1412-2</a>) at 1/1,000 dilution.<br /><br />Lane 1: recombinant murine Interleukin-6 protein<br />.<br />Exposure time: 30 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1412-2" style="font-weight: bold;text-decoration: underline;">R1412-2</a>, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 23.7 kDa<br />Observed band size: 22 kDa

    Western blot analysis of IL-6 on different lysates with Rabbit anti-IL-6 antibody (R1412-2) at 1/1,000 dilution.

    Lane 1: recombinant murine Interleukin-6 protein
    .
    Exposure time: 30 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1412-2, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 23.7 kDa
    Observed band size: 22 kDa

文献・引用論文

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

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