Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

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Catalog# EHY0073

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

Application

ELISA

Reactivity

Human

Standard curve range

0.01~10 μg/ml

製品概要

製品名

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

製品タイプ

Elisa Kit

検出感度

0.14 ng/ml (10 μl)

μg/ml	O.D.		Average	Corrected
0.00	0.0095	0.0098	0.0097
0.01	0.0453	0.0436	0.0445	0.0348
0.04	0.1151	0.1129	0.1140	0.1044
0.12	0.3329	0.3291	0.3310	0.3214
0.37	0.9104	0.9296	0.9200	0.9104
1.11	2.2030	2.2310	2.2170	2.2074
3.33	3.8400	3.8250	3.8325	3.8229
10.00	4.3491	4.4561	4.4026	4.3930

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (μg/ml)	0.2	1.1	3.7	0.2	1.2	3.5
Standard Deviation	0.0	0.1	0.2	0.1	0.1	0.2
Coefficient of Variation (%)	5.1	5.0	5.3	5.2	4.9	5.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human Clusterin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 86%.

サンプル値

Sample Matrix	Sample Evaluated	Range (μg/ml)	Detectable (%)	Mean of Detectable (μg/ml)
Human Serum	30	94.12-210.07	100	142.61
Monkey Serum	2	96.75-108.47	100	102.61

Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human Clusterin in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

60min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P10909 Human

別名

AAG 4

AAG4

Aging associated protein 4

Aging-associated gene 4 protein

AI893575

APO J

Apo-J

APOJ

ApoJalpha

展開

機能

Clusterin [also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40], is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis. Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, brain, and in many epithelial tissues during embryonic development. Human Clusterin is synthesized as a precursor that contains two coiled coil domains, three nuclear localization signals (NLS), and one heparin binding domain. Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and beta chains which are secreted as an approximately 80 kDa N-glycosylated and disulfide-linked heterodimer. Mature human Clusterin shares a 77% amino acid sequence identity with mouse and rat Clusterin. High μg/mL concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin. The ability of Clusterin to bind and neutralize non-oxidatively modified LDL reduces cytotoxicity in atherosclerotic plaques. The chaperone function of Clusterin helps to reduce the accumulation of beta -amyloid fibrils and damage due to amyloid plaques in Alzheimer's disease. An alternately spliced 50 kDa isoform of human Clusterin (nCLU) remains intracellular and is neither glycosylated nor cleaved into alpha and beta chains. Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis. This function contrasts with the cytoprotective effect of secreted Clusterin. During tumor progression, nCLU is down regulated while the secreted form is upregulated and may be aberrantly glycosylated. Increased circulating levels of Clusterin enhance tumor aggressiveness by inhibiting apoptosis and by promoting the epithelial to mesenchymal transition.

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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