IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35] - BSA and Azide free
Catalog# HA752016
IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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ET1704-77
含抗保成分
-
unconjugated
製品概要
製品名
IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35] - BSA and Azide free
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human IP3 Receptor aa 2190-2280 / 2758.
交差反応性（対応種属）
Human, Mouse, Rat
検証済みアプリケーション
WB, IHC-P, IHC-Fr, IF-Tissue
標的分子量
Predicted band size: 314 kDa
ポジティブコントロール
Rat brain tissue lysate, Mouse brain tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.
コンジュゲーション
unconjugated
クローン番号
JA11-35
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways.
背景・参考文献
1. Cao X. et. al. ORP4L couples IP(3) to ITPR1 in control of endoplasmic reticulum calcium release. FASEB J. 2019 Dec
2. Synofzik M. et. al. De novo ITPR1 variants are a recurrent cause of early-onset ataxia, acting via loss of channel function. Eur J Hum Genet. 2018 Nov
配列相同性
Belongs to the InsP3 receptor family.
組織特異性
Widely expressed.
翻訳後修飾（PTM）
Phosphorylated on tyrosine residues.; Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity).; Phosphorylated by cAMP kinase (PKA). Phosphorylation prevents the ligand-induced opening of the calcium channels. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1.; Palmitoylated by ZDHHC6 in immune cells, leading to regulate ITPR1 stability and function.
サブセルラー局在
Endoplasmic reticulum membrane, secretory vesicle membrane, perinuclear region.
別名
4 antibody
5-trisphosphate receptor antibody
5-trisphosphate receptor type 1 antibody
DKFZp313E1334 antibody
DKFZp313N1434 antibody
inositol 1 4 5 triphosphate receptor type 1 antibody
Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
Inositol 1 antibody
InsP3R1 antibody
IP3 antibody
詳細を見る画像
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This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Western blot analysis of IP3 Receptor on different lysates with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 314 kDa
Observed band size: 314 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
This data was developed using ET1704-77, the same antibody clone in a different buffer formulation.
Application: IF-Tissue
Species: Rat
Site: cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
同一ターゲット＆同一シグナル伝達経路の関連製品
IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35]
Application: WB,IHC-P,IHC-Fr,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
