Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-66] - BSA and Azide free
Catalog# HA751527
Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-66] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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HA723664
含抗保成分
-
unconjugated
概要
製品名
Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-66] - BSA and Azide free
抗体の種類
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Granzyme B aa 1-247.
種属反応性
Human, Mouse
検証された応用例
WB, IHC-P
分子量
Predicted band size: 28 kDa
陽性対照
NK-92 cell lysate, CTLL-2 cell lysate, human NK/T-cell lymphoma tissue, human spleen tissue.
結合
unconjugated
クローン番号
PSH14-66
製品の特徴
形態
Liquid
保存方法
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
保存用バッファー
PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
応用希釈度
-
WB
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1:100,000
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IHC-P
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1:500-1:5,000
ターゲット
機能
Granzyme B (GrB) is one of the serine protease granzymes most commonly found in the granules of natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells. Granzyme B has also been found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. The secondary functions of granzyme B are also numerous. Granzyme B has shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodelling. Elevated levels of granzyme B are also implicated in a number of autoimmune diseases, several skin diseases, and type 1 diabetes.
背景文献
1. Gleave A et al. Granzyme B in Autoimmune Skin Disease. Biomolecules. 2023 Feb
2. Jung K et al. Granzyme B in epithelial barrier dysfunction and related skin diseases. Am J Physiol Cell Physiol. 2022 Jul
亜細胞局在
Secreted, Cytolytic granule.
別名
C11 antibody
Cathepsin G like 1 antibody
Cathepsin G-like 1 antibody
CCPI antibody
CGL 1 antibody
CGL1 antibody
CSP B antibody
CSPB antibody
CTLA 1 antibody
CTLA-1 antibody
展開画像
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☑ Relative expression (RE)
Western blot analysis of Granzyme B on different lysates with Rabbit anti-Granzyme B antibody (HA751527) at 1/100,000 dilution.
Lane 1: NK-92 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (negative) (20 µg/Lane)
Lane 3: CTLL-2 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (negative) (20 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 30 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751527) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue with Rabbit anti-Granzyme B antibody (HA751527) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751527) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Granzyme B antibody (HA751527) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751527) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"