SOX1 Recombinant Rabbit Monoclonal Antibody [PSH10-51] - BSA and Azide free
Catalog# HA751354
SOX1 Recombinant Rabbit Monoclonal Antibody [PSH10-51] - BSA and Azide free
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WB
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IHC-Fr
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IHC-P
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Human
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Mouse
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Rat
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HA723215
含抗保成分
-
Cynomolgus monkey
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Pig
予測される交差反応性
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unconjugated
製品概要
製品名
SOX1 Recombinant Rabbit Monoclonal Antibody [PSH10-51] - BSA and Azide free
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human SOX1 aa 1-391.
交差反応性（対応種属）
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される交差反応性
検証済みアプリケーション
WB, IHC-Fr, IHC-P
標的分子量
Predicted band size: 39 kDa
ポジティブコントロール
Human cerebellum tissue, mouse cerebellum tissue, mouse E14.5 embryonic eyeball tissue, rat cerebellum tissue, rat E14.5 embryo tissue, NCCIT cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
コンジュゲーション
unconjugated
クローン番号
PSH10-51
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
1*PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
背景・参考文献
1. Sun X et al. Anti-SOX1 Antibodies in Paraneoplastic Neurological Syndrome. J Clin Neurol. 2020 Oct
2. Kanwore K et al. SOX1 Is a Backup Gene for Brain Neurons and Glioma Stem Cell Protection and Proliferation. Mol Neurobiol. 2021 Jun
サブセルラー局在
Nucleus.
別名
Sex determining region Y box 1 antibody
sox1 antibody
SOX1_HUMAN antibody
SRY antibody
SRY box containing gene 1 antibody
SRY related HMG box gene 1 antibody
Transcription factor Sox-1 antibody
画像
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This data was developed using HA723215, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryonic brain
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using HA723215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-SOX1 antibody (HA723215) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SOX1 antibody (HA723215) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryonic eyeball tissue with Rabbit anti-SOX1 antibody (HA723215) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SOX1 antibody (HA723215) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-SOX1 antibody (HA723215) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723215, the same antibody clone in a different buffer formulation.
Western blot analysis of SOX1 on different lysates with Rabbit anti-SOX1 antibody (HA723215) at 1/2,000 dilution.
Lane 1: NCCIT cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723215) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）