TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89] - BSA and Azide free

This data was developed using HA722195, the same antibody clone in a different buffer formulation.
Application: IHC-Fr

Species: Mouse

Site: Raphe nucleus

Sample: Frozen section

Antibody concentration: 1/500

Antigen retrieval: Not required

This data was developed using HA722195, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse midbrain tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA722195, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA722195, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

This data was developed using HA722195, the same antibody clone in a different buffer formulation.
Western blot analysis of TPH2 on different lysates with Rabbit anti-TPH2 antibody (HA722195) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Mouse kidney tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 50 kDa

Exposure time: 1 minute 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722195) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA722195, the same antibody clone in a different buffer formulation.
TPH2 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA722195 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722195 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: rat brain tissue lysate (input)
Lane 2: HA722195 IP in rat brain tissue lysate
Lane 3: Rabbit IgG instead of HA722195 in rat brain tissue lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801