APOE4 Recombinant Rabbit Monoclonal Antibody [PSH02-85] - BSA and Azide free
Catalog# HA750838
APOE4 Recombinant Rabbit Monoclonal Antibody [PSH02-85] - BSA and Azide free
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WB
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Human
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HA721867
含抗保成分
-
unconjugated
製品概要
製品名
APOE4 Recombinant Rabbit Monoclonal Antibody [PSH02-85] - BSA and Azide free
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Apolipoprotein E aa 101-150 / 317.
交差反応性（対応種属）
Human
検証済みアプリケーション
WB
標的分子量
Predicted band size: 36 kDa
コンジュゲーション
unconjugated
クローン番号
PSH02-85
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
1*PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
Apolipoprotein-E (apoE) is a protein component of plasma lipoproteins that mediates the binding, internalization and catabolism of lipoprotein particles. It can serve as a ligand for several lipoprotein receptors, including the LDL (ApoB/E) receptor and the hepatic apoE (chylomicron remnant) receptor. apoE is produced in most organs and occurs in all plasma lipoprotein fractions, constituting 10-20% of VLDL (very low density lipoprotein) and 1-2% of HDL (high density lipoprotein). Three major isoforms of apoE have been described in human (E2, E3 and E4) which differ by only one or two amino acids. Estrogen receptor has been shown to upregulate apoE gene expression via the ERa-mediated pathway, indicating a potential role for apoE in atherosclerosis. This is consistent with studies in mice in which plasma apoE levels were raised, thereby protecting the mice from diet-induced atherosclerosis. apoE has also been shown to be a potent inhibitor of proliferation and thus may play a role in angiogenesis, tumor cell growth and metastasis.
背景・参考文献
1. Yang LG et al. Apolipoprotein E in lipid metabolism and neurodegenerative disease. Trends Endocrinol Metab. 2023 Aug
2. Saito T et al. Apolipoprotein E-related glomerular disorders. Kidney Int. 2020 Feb
サブセルラー局在
Secreted, extracellular space, extracellular matrix.
UNIPROT #
別名
AD2 antibody
Apo-E antibody
APOE antibody
APOE_HUMAN antibody
APOEA antibody
Apolipoprotein E antibody
ApolipoproteinE antibody
Apoprotein antibody
LDLCQ5 antibody
LPG antibody
詳細を見る画像
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☑ Cell treatment (CT)
This data was developed using HA721867, the same antibody clone in a different buffer formulation.
Western blot analysis of APOE4 on different lysates with Rabbit anti-APOE4 antibody (HA721867) at 1/2,000 dilution.
Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-APOE2 cell lysate
Lane 3: 293T-OE-APOE3 cell lysate
Lane 4: 293T-OE-APOE4 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 2 minutes 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721867) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using HA721867, the same antibody clone in a different buffer formulation.
Western blot analysis of APOE4 on different lysates with Rabbit anti-APOE4 antibody (HA721867) at 1/2,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD APOE4 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721867) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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