Human FAP Recombinant Rabbit Monoclonal Antibody [PSH26-55] - BSA and Azide free (Capture)
製品概要
製品名
Human FAP Recombinant Rabbit Monoclonal Antibody [PSH26-55] - BSA and Azide free (Capture)
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human FAP aa 26-760 (HA211419).
交差反応性（対応種属）
Human
検証済みアプリケーション
ELISA(Cap)
ポジティブコントロール
Recombinant Human FAP protein (HA211419).
コンジュゲーション
unconjugated
クローン番号
PSH26-55
Reactivity Data
検証済（社内検証通過）
文献報告済（社内未検証、文献サポートあり）
反応性予測（高い相同性に基づく）
非推奨（社内検証未通過）
| Elisa | |
|---|---|
| human |
|
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
1*PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-56] to Human FAP (Detecor) (HA725500) and Recombinant Human FAP protein (HA211419) as the standard. The reference range value is 156.3-10,000 pg/mL.
ターゲット
機能
FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is co-localized with MMP-1and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.
背景・参考文献
1. Jia, B. et al. 2016. GPR30 Promotes Prostate Stromal Cell Activation via Suppression of ERα Expression and Its Downstream Signaling Pathway. Endocrinology. 157: 3023-35.
2. Knopf, JD. et al. 2015. The stromal cell-surface protease fibroblast activation protein-α localizes to lipid rafts and is recruited to invadopodia. Biochim. Biophys. Acta. 1853: 2515-25.
サブセルラー局在
Cell membrane. Cell surface.
UNIPROT #
別名
170 kDa melanoma membrane bound gelatinase antibody
170 kDa melanoma membrane-bound gelatinase antibody
DPPIV antibody
FAP antibody
FAPA antibody
Fibroblast activation protein alpha antibody
Integral membrane serine protease antibody
SEPR_HUMAN antibody
Seprase antibody
画像
-
![Sandwich ELISA analysis of Human FAP matched pair antibodies<br /><br />Capture: <a href="/products/HA725499" style="font-weight: bold;text-decoration: underline;">HA725499</a>, Human FAP Rabbit mAb [PSH26-55]<br />Detector: <a href="/products/HA725500" style="font-weight: bold;text-decoration: underline;">HA725500</a>, Human FAP Rabbit mAb [PSH26-56]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725499" style="font-weight: bold;text-decoration: underline;">HA725499</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAP protein (<a href="/products/HA211419" style="font-weight: bold;text-decoration: underline;">HA211419</a>) starting from 10,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA725500" style="font-weight: bold;text-decoration: underline;">HA725500</a>, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725499_1.png?v=20260908000743)
Sandwich ELISA analysis of Human FAP matched pair antibodies
Capture: HA725499, Human FAP Rabbit mAb [PSH26-55]
Detector: HA725500, Human FAP Rabbit mAb [PSH26-56]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725499) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAP protein (HA211419) starting from 10,000 pg/mL to 0 pg/mL and detect antibody (HA725500, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native FAP in human serum.<br /><br />Capture: <a href="/products/HA725499" style="font-weight: bold;text-decoration: underline;">HA725499</a>, Human FAP Rabbit mAb [PSH26-55]<br />Detector: <a href="/products/HA725500" style="font-weight: bold;text-decoration: underline;">HA725500</a>, Human FAP Rabbit mAb [PSH26-56]<br /><br />Interpolated concentration of native FAP was measured in duplicate at different sample concentrations and interpolated from the FAP standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAP concentration was determined to be 69,308 pg/mL in human serum.](https://storage.huabio.cn/huabio/productImg/HA725499_2.png?v=20260908000743)
Interpolated concentrations of native FAP in human serum.
Capture: HA725499, Human FAP Rabbit mAb [PSH26-55]
Detector: HA725500, Human FAP Rabbit mAb [PSH26-56]
Interpolated concentration of native FAP was measured in duplicate at different sample concentrations and interpolated from the FAP standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAP concentration was determined to be 69,308 pg/mL in human serum.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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