HRP Conjugated Human/Monkey Albumin Recombinant Rabbit Monoclonal Antibody [PSH20-17] - Detector
製品概要
製品名
HRP Conjugated Human/Monkey Albumin Recombinant Rabbit Monoclonal Antibody [PSH20-17] - Detector
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Albumin.
交差反応性（対応種属）
Human, Dog, Cynomolgus monkey
検証済みアプリケーション
ELISA(Det), ELISA
ポジティブコントロール
Human Albumin protein (HA211532).
コンジュゲーション
HRP
クローン番号
PSH20-17
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-16] to Human/Monkey Albumin antibody (Capture) (HA724127) and Human Albumin protein (HA211532) as the standard. The reference range value is 39.06-5,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
ターゲット
機能
Serum albumin (ALB), the main protein in plasma, has a very good binding capacity for water, fatty acids, calcium, sodium, bilirubin, hormones, potassium and drugs. The primary function of ALB is to regulate the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted form of albumin. Mutations in the ALB gene may result in familial dysalbuminemic hyperthyroxinemia (FDH), a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T4. FDH is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian populations.
背景・参考文献
1. Zhai W et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016).
2. Ma J et al. Pramipexole-Induced Hypothermia Reduces Early Brain Injury via PI3K/AKT/GSK3 pathway in Subarachnoid Hemorrhage rats. Sci Rep 6:23817 (2016).
サブセルラー局在
Secreted.
UNIPROT #
別名
alb antibody
ALBU_HUMAN antibody
Albumin (32 AA) antibody
Albumin (AA 34) antibody
cell growth inhibiting protein 42 antibody
DKFZp779N1935 antibody
GIG20 antibody
GIG42 antibody
growth-inhibiting protein 20 antibody
OTTHUMP00000220436 antibody
詳細を見る画像
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![Sandwich ELISA analysis of Human Albumin matched pair antibodies<br /><br />Capture: <a href="/products/HA724127" style="font-weight: bold;text-decoration: underline;">HA724127</a>, Human/Monkey Albumin Rabbit mAb [PSH20-16]<br />Detector: <a href="/products/HA724128" style="font-weight: bold;text-decoration: underline;">HA724128</a>, Human/Monkey Albumin Rabbit mAb [PSH20-17]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA724127" style="font-weight: bold;text-decoration: underline;">HA724127</a>) diluted in carbonate/bicarbonate buffer, at a concentHumanion of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Albumin protein (<a href="/products/HA211532" style="font-weight: bold;text-decoration: underline;">HA211532</a>) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA724128" style="font-weight: bold;text-decoration: underline;">HA724128</a>, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstHumane for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA724128H_1.jpg?v=20260604174659)
Sandwich ELISA analysis of Human Albumin matched pair antibodies
Capture: HA724127, Human/Monkey Albumin Rabbit mAb [PSH20-16]
Detector: HA724128, Human/Monkey Albumin Rabbit mAb [PSH20-17]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724127) diluted in carbonate/bicarbonate buffer, at a concentHumanion of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Albumin protein (HA211532) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724128, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstHumane for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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