Choline Acetyltransferase Recombinant Rabbit Monoclonal Antibody [PSH15-49]
Catalog# HA723755
Choline Acetyltransferase Recombinant Rabbit Monoclonal Antibody [PSH15-49]
-
IHC-P
-
Mouse
-
Rat
-
HA751564
不含抗保成分
-
unconjugated
製品概要
製品名
Choline Acetyltransferase Recombinant Rabbit Monoclonal Antibody [PSH15-49]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse Choline Acetyltransferase aa 1-641.
交差反応性（対応種属）
Mouse, Rat
検証済みアプリケーション
IHC-P
分子量
Predicted band size: 72 kDa
ポジティブコントロール
Mouse brain (habenular nucleus) tissue, mouse brain (caudate nucleus) tissue, mouse hindbrain tissue, rat brain (habenular nucleus) tissue, rat brain (caudate nucleus) tissue, rat hindbrain tissue.
コンジュゲーション
unconjugated
クローン番号
PSH15-49
Reactivity Data
| IHC-P | |
|---|---|
| Mouse |
|
| Rat |
|
| Human |
|
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
IHC-P
-
1:200
ターゲット
機能
Choline acetyltransferase (also designated choactase, choline O-acetyltransferase) synthesizes acetylcholine in cholinergic neurons. Multiple choactase mRNAs with different 5'-noncoding regions are expressed as R-, N1, N2-, S- and M-types. N1-, N2- and R-type mRNAs produce a single short enzyme, while M-type mRNA produces both long and short enzymes. The long enzyme is targeted to the nuclei of cells, whereas the short protein is found in cytoplasm. A novel NFkB binding site is located within the nerve growth factor-responsive enhancer element that is recognized by the NFkB protein p49, but not p65 or p50. Decreased choactase expression and increased NFkB activity are associated with aging and Alzheimer's disease, indicating that p49 is a negative regulator of choactase expression and suggesting a possible mechanism for aging-associated declines in cholinergic function. Phosphorylation of choactase has been shown to enhance choactase catalytic activity. Specifically, Serine 440 is found to be the phosphorylation site in a recombinant human short choactase by protein kinase C and is involved in regulation of the enzyme catalytic activity and binding to subcellular membranes.
背景・参考文献
1. Gabalski AH et al. Circulating extracellular choline acetyltransferase regulates inflammation. J Intern Med. 2024 Mar
2. Liu J et al. Choline acetyltransferase and vesicular acetylcholine transporter are required for metamorphosis, reproduction, and insecticide susceptibility in Tribolium castaneum. Gene. 2022 Oct
サブセルラー局在
Cytosol, nucleus, cytoplasm, neuron projection, presynapse.
UNIPROT #
別名
Acetyl CoA choline O acetyltransferase antibody
Acetyl CoA:choline O acetyltransferase antibody
ChAT antibody
CHOACTase antibody
Choline acetylase antibody
choline acetyltransferase antibody
Choline O acetyltransferase antibody
Choline O-acetyltransferase antibody
CLAT_HUMAN antibody
CMS1A antibody
詳細を見る画像
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Immunohistochemical analysis of paraffin-embedded mouse brain (habenular nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA723755) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723755) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain (caudate nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA723755) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723755) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse hindbrain tissue with Rabbit anti-Choline Acetyltransferase antibody (HA723755) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723755) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain (habenular nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA723755) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723755) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain (caudate nucleus) tissue with Rabbit anti-Choline Acetyltransferase antibody (HA723755) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723755) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat hindbrain tissue with Rabbit anti-Choline Acetyltransferase antibody (HA723755) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723755) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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