iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
Catalog# HA720153F
iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
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IF-Cell
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FC
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Human
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iFluor™ 488
製品概要
製品名
iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Calretinin aa 60-100.
交差反応性（対応種属）
Human
検証済みアプリケーション
IF-Cell, FC
標的分子量
Predicted band size: 32 kDa
ポジティブコントロール
SH-SY5Y.
コンジュゲーション
iFluor™ 488
クローン番号
JM12-93
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存バッファー
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
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IF-Cell
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1:100
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FC
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1:500-1:1,000
ターゲット
機能
Calbindin D28K and Calretinin (also designated CR or 29 kDa Calbindin) are two closely related intracellular calcium-binding proteins belonging to the Troponin-C superfamily. Initially isolated from chick retina, Calretinin shares 58% identical residues with human Calbindin D28K. Calretinin is expressed in the brain and is particularly abundant in auditory neurons with precisely timed discharges. Neurons in the nucleus accumbens containing Calretinin all possess nuclear indentations. Calretinin-immunoreactive boutons form asymmetrical and symmetrical synaptic specializations on spines, dendrites and somata. The symmetrical synaptic specializations have medium-sized spiny neurons and contact other Calretinin-immunoreactive somata. Calretinin is widely used as a immunocytochemical marker for mesothelioma.
背景・参考文献
1. Francavilla C et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017).
2. McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016).
サブセルラー局在
Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.
UNIPROT #
別名
29 kDa calbindin antibody
CAB 29 antibody
CAB29 antibody
CAL 2 antibody
CAL2 antibody
CALB 2 antibody
CALB2 antibody
CALB2_HUMAN antibody
Calbindin 2 29kDa antibody
Calbindin 2 antibody
詳細を見る画像
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Immunocytochemistry analysis of SH-SY5Y cells labeling Calretinin with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. -
Flow cytometric analysis of SH-SY5Y cells labeling Calretinin.
Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Calretinin (HA720153F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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