SOX2 Recombinant Antibody [PO00-28] - Chicken IgY (Chimeric)
Catalog# HA601499
SOX2 Recombinant Antibody [PO00-28] - Chicken IgY (Chimeric)
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IHC-Fr
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WB
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Human
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Mouse
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HA610329
不含抗保成分
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unconjugated
製品概要
製品名
SOX2 Recombinant Antibody [PO00-28] - Chicken IgY (Chimeric)
抗体のタイプ
Recombinant Chimeric Antibody
免疫原
Recombinant protein within human SOX2 aa 1-317.
交差反応性（対応種属）
Human, Mouse
検証済みアプリケーション
IHC-Fr, WB
標的分子量
Predicted band size: 34 kDa
ポジティブコントロール
Mouse cerebral cortex, NCCIT cell lysate, F9 cell lysate.
コンジュゲーション
unconjugated
クローン番号
PO00-28
Reactivity Data
検証済（社内検証通過）
文献報告済（社内未検証、文献サポートあり）
反応性予測（高い相同性に基づく）
非推奨（社内検証未通過）
| IHC-Fr | |
|---|---|
| mouse |
|
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgY
精製方法
Protein A affinity purified.
推奨希釈倍率
-
IHC-Fr
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1:200
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WB
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1:5,000
ターゲット
機能
The differentiation of seminomas from non-seminomatous germ cell tumors can be challenging, especially, if small biopsy specimens, necrotic tumors and metastatic tumors with artifacts are encountered. A subset of germ cell tumors may require immunohistochemistry (IHC) for classification owing to unusual morphologic features, such as diffuse growth of clear cells, and tumors with glandular and/or microcytic patterns. In the mixed germ cell tumor, one component is often intermingled intimately with others such as embryonal carcinoma versus yolk sac tumor, can be overlooked. IHC will identify such an area and allow for the identification of each component of the mixed tumor more accurately and documenting them in the pathology report is recommended by WHO. Current IHC studies have shown the combination of CD30/CD117 staining plays a good role in distinguishing between embryonal carcinoma and yolk sac tumor. However, a subset of tumors may not be distinguished by this combination. Also, the characteristic membranous pattern by antibodies to CD30 and CD117 for the interpretation of the diagnosis may not be evident in limited biopsy specimens. In this respect, transcription factors, such as SOX-2, are easier to interpret due to their distinct nuclear reaction. SOX-2 has been reported as a diagnostic marker for embryonal carcinoma. SOX-2 was expressed in intratubular embryonal carcinoma, pure embryonal carcinoma and in the embryonal carcinoma component of mixed germ cell tumor in all cases. But, SOX-2 expression has not been found in seminoma, yolk sac tumor, and choriocarcinoma in almost all cases.
背景・参考文献
1. Novak D. et. al. SOX2 in development and cancer biology. Semin Cancer Biol. 2020 Dec
2. Porter L. et. al. SOX2 and squamous cancers. Semin Cancer Biol. 2020 Dec
サブセルラー局在
Nucleus.
別名
ANOP3 antibody
cb236 antibody
Delta EF2a antibody
lcc antibody
MCOPS3 antibody
MGC148683 antibody
MGC2413 antibody
RGD1565646 antibody
Sex determining region Y box 2 antibody
SOX 2 antibody
詳細を見る画像
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Application: IHC-Fr
Species: Mouse
Site: cerebral cortex
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Western blot analysis of SOX2 on different lysates with Chicken anti-SOX2 antibody (HA601499) at 1/5,000 dilution.
Lane 1: NCCIT cell lysate
Lane 2: F9 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 36 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601499) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Chicken IgY - HRP Secondary Antibody (HA1046) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）