Nestin Recombinant Antibody [PSH07-89] - Guinea pig IgG2 (Chimeric)
Catalog# HA601468
Nestin Recombinant Antibody [PSH07-89] - Guinea pig IgG2 (Chimeric)
-
IHC-Fr
-
WB
-
Human
-
Mouse
-
Rat
概要
製品名
Nestin Recombinant Antibody [PSH07-89] - Guinea pig IgG2 (Chimeric)
抗体の種類
Recombinant Chimeric Antibody
免疫原
Recombinant protein within mouse Nestin aa 700-1,000.
種属反応性
Human, Mouse, Rat
検証された応用例
IHC-Fr, WB
分子量
Predicted band size: 207 kDa
陽性対照
RD cell lysate, C2C12 cell lysate, C6 cell lysate.
結合
unconjugated
クローン番号
PSH07-89
製品の特徴
形態
Liquid
濃度
1ug/ul
保存方法
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
精製方法
Protein A affinity purified.
応用希釈度
-
IHC-Fr
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1:200
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WB
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1:10,000
ターゲット
機能
Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
背景文献
1. Chen H et al. Targeting Nestin(+) hepatic stellate cells ameliorates liver fibrosis by facilitating TbetaRI degradation. J Hepatol. 2021 May
2. Wang J et al. Nestin promotes pulmonary fibrosis via facilitating recycling of TGF-beta receptor I. Eur Respir J. 2022 May
亜細胞局在
Cytoplasm, Intermediate filament.
別名
ESTM 46 antibody
FLJ 21841 antibody
FLJ21841 antibody
Intermediate filament protein antibody
Nbla00170 antibody
nes antibody
NEST_HUMAN antibody
Nestin antibody
画像
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Application: IHC-Fr
Species: Mouse
Site: embryonic cartilage
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: kidney
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: kidney
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
☑ Relative expression (RE)
Western blot analysis of Nestin on different lysates with Guinea pig anti-Nestin antibody (HA601468) at 1/10,000 dilution.
Lane 1: RD cell lysate
Lane 2: 293T cell lysate (negative)
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 207 kDa
Observed band size: 100-300 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601468) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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