Claudin 18.2 Recombinant Mouse Monoclonal Antibody [PSH18-20]
Catalog# HA601432
Claudin 18.2 Recombinant Mouse Monoclonal Antibody [PSH18-20]
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IHC-P
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Human
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unconjugated
製品概要
製品名
Claudin 18.2 Recombinant Mouse Monoclonal Antibody [PSH18-20]
抗体のタイプ
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein.
交差反応性（対応種属）
Human
検証済みアプリケーション
IHC-P
標的分子量
Predicted band size: 28 kDa
ポジティブコントロール
Human stomach cancer tissue.
コンジュゲーション
unconjugated
クローン番号
PSH18-20
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
IHC-P
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1:50-1:200
ターゲット
機能
CLDN-18 has two isoforms, CLDN-18.1 and CLDN-18.2, that are expressed mainly in normal lung and gastric tissues, respectively. CLDN-18 is expressed in many neoplasias, such as gastric and gastroesophageal junction (G/GEJ) cancer, pancreatic cancer, head and neck cancer and non-small cell lung cancer. Different therapeutic drugs targeting CLDN-18.2 for cancer immunotherapy have now been developed and IHC for CLDN-18.2 to be used for the selection of patients to be offered anti-CLDN18.2 treatment. The distinction and scoring criteria separating CLDN-18.2 positive cancers versus negative cancers depend on tumour type and therapeutic agent. For G/GEJ cancer a moderate to strong (2+/3+) positive membrane staining in ≥75% of tumor cells should be considered as the standard cut-off for a CLDN18.2-positive tumor to be treated with zolbetuximab (VYLOY™, Astellas). CLDN-18.2 has a very limited expression in normal tissues and is exclusively expressed in the mucosal membrane of differentiated gastric epithelial cells complicating the design of guidelines for composition of control material for CLDN-18.2 IHC. The gastric epithelial cells will typically show a moderate to strong membranous staining reaction and thus mainly applicable to confirm “right IHC assay” being applied. No staining reaction should be seen in e.g. smooth muscle cells, lymphocytes and endothelial cells enabling these structures to be used as negative tissue controls. Gastric intestinal metaplasia is reported and shown to have a reduced CLDN-18.2 expression and potential to be used as immunohistochemical critical assay performance control (iCAPC) with emphasis on low level demonstration of CLDN-18.2. Gastric intestinal metaplastic epithelial cells (either as external tissue control or internally in patient sample) will typically show a weak to moderate staining reaction. At present internal NordiQC testing has revealed that the presence of gastric intestinal metaplasia is very focal and heterogeneously observed compromising the use for iCAPC in external tissue controls.
背景・参考文献
1. Nakayama I et al. Claudin 18.2 as a novel therapeutic target. Nat Rev Clin Oncol. 2024 May
2. Kubota Y et al. Comprehensive clinical and molecular characterization of claudin 18.2 expression in advanced gastric or gastroesophageal junction cancer. ESMO Open. 2023 Feb
サブセルラー局在
Cell junction, tight junction, Lateral cell membrane.
UNIPROT #
別名
Claudin 18 antibody
Claudin-18 antibody
CLD18_HUMAN antibody
CLDN18 antibody
SFTA5 antibody
SFTPJ antibody
CLDN18.2
画像
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Claudin 18.2 antibody (HA601432) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601432) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Claudin 18.2 antibody (HA601432) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601432) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Claudin 18.2 antibody (HA601432) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601432) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Claudin 18.2 antibody (HA601432) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601432) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）