NF-M Recombinant Antibody [JM11-20] - Rat IgG1 (Chimeric)
Catalog# HA601408
NF-M Recombinant Antibody [JM11-20] - Rat IgG1 (Chimeric)
-
IHC-Fr
-
IHC-P
-
WB
-
Human
-
Mouse
-
Rat
-
HA610248
不含抗保成分
-
Cynomolgus monkey
予測される交差反応性
-
unconjugated
製品概要
製品名
NF-M Recombinant Antibody [JM11-20] - Rat IgG1 (Chimeric)
抗体のタイプ
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within Human NEFM aa 651-692 / 916.
交差反応性（対応種属）
Human, Mouse, Rat (Predicted: Cynomolgus monkey)
予測される交差反応性
検証済みアプリケーション
IHC-Fr, IHC-P, WB
標的分子量
Predicted band size: 102 kDa
ポジティブコントロール
Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
コンジュゲーション
unconjugated
クローン番号
JM11-20
Reactivity Data
| IHC-Fr | IHC-P | WB | |
|---|---|---|---|
| Human |
|
||
| Mouse |
|
|
|
| Rat |
|
|
|
| Cynomolgus Monkey |
|
|
|
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
精製方法
Protein A affinity purified.
推奨希釈倍率
-
IHC-Fr
-
1:500
-
IHC-P
-
1:500
-
WB
-
1:5,000-1:10,000
ターゲット
機能
Neurofilament-M (NF-M), for neurofilament medium polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.
背景・参考文献
1. Li D et al. NEFM DNA methylation correlates with immune infiltration and survival in breast cancer. Clin Epigenetics. 2021 May
2. Sohrabi N et al. Regulatory Role of Insulin on Endogenous L1 ORF1 and NEFM Gene Expression through PI3K Signaling Pathway Specifically in Neuroblastoma Cell Line. Iran J Public Health. 2023 Mar
サブセルラー局在
Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.
別名
150kDa medium antibody
NEF3 antibody
NEFM antibody
Neurofilament 3 antibody
NF160 antibody
NFM antibody
画像
-
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-NF-M antibody (HA601408) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601408) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-M antibody (HA601408) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601408) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NF-M antibody (HA601408) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601408) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of NF-M on different lysates with Rat anti-NF-M antibody (HA601408) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 140 kDa
Exposure time: 23 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601408) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）