VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)
Catalog# HA601393
VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)
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IHC-Fr
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WB
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Mouse
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Rat
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Cynomolgus monkey
-
Pig
予測される種属に対するアフターサービスのサポート
概要
製品名
VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)
抗体の種類
Recombinant Chimeric Antibody
種属反応性
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される種属に対するアフターサービスのサポート
検証された応用例
IHC-Fr, WB
分子量
Predicted band size: 65 kDa
陽性対照
Mouse cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
結合
unconjugated
クローン番号
PSH10-54
製品の特徴
形態
Liquid
濃度
1ug/ul
保存方法
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG1
精製方法
Protein A affinity purified.
応用希釈度
-
IHC-Fr
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1:500
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WB
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1:2,000
ターゲット
機能
Predicted to enable L-glutamate transmembrane transporter activity and neurotransmitter transmembrane transporter activity. Involved in neurotransmitter loading into synaptic vesicle. Predicted to be located in synaptic vesicle. Predicted to be active in excitatory synapse. Predicted to be integral component of synaptic vesicle membrane.
背景文献
1. Buck SA et al. Roles of VGLUT2 and Dopamine/Glutamate Co-Transmission in Selective Vulnerability to Dopamine Neurodegeneration. ACS Chem Neurosci. 2022 Jan
2. Zhang Y et al. VGluT2 neuron subtypes in the paraventricular thalamic nucleus regulate depression in paraquat-induced Parkinson\'s disease. J Hazard Mater. 2024 Jul
亜細胞局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane.
UNIPROT #
別名
Differentiation associated BNPI antibody
Differentiation associated Na dependent inorganic phosphate cotransporter antibody
Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
Differentiation-associated BNPI antibody
Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
DNPI antibody
SLC17A6 antibody
Sodium dependent inorganic phosphate cotransporter antibody
Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
Solute carrier family 17 member 6 antibody
展開画像
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
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☑ Relative expression (RE)
Western blot analysis of VGLUT2 on different lysates with Rat anti-VGLUT2 antibody (HA601393) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse lung tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat lung tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601393) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"