Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29]
Catalog# HA601369
Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29]
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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IP
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mIHC
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Mouse
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Rat
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HA610215
不含抗保成分
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unconjugated
製品概要
製品名
Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29]
抗体のタイプ
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within human Calretinin aa 1-200.
交差反応性（対応種属）
Mouse, Rat
検証済みアプリケーション
WB, IHC-P, IHC-Fr, IF-Tissue, IP, mIHC
標的分子量
Predicted band size: 32 kDa
ポジティブコントロール
Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate, mouse brain tissue, rat brain tissue.
コンジュゲーション
unconjugated
クローン番号
PSH08-29
Reactivity Data
検証済（社内検証通過）
文献報告済（社内未検証、文献サポートあり）
反応性予測（高い相同性に基づく）
非推奨（社内検証未通過）
| WB | IHC-P | IHC-Fr | IF-Tissue | IP | mIHC | |
|---|---|---|---|---|---|---|
| mouse |
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| rat |
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製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG1
精製方法
Protein A affinity purified.
推奨希釈倍率
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WB
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1:2,000
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IHC-P
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1:1,000-1:2,000
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IHC-Fr
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1:500
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IF-Tissue
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1:500
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mIHC
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1:1,000
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IP
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1-2μg/sample
ターゲット
機能
Calretinin, also known as calbindin 2 (formerly 29 kDa calbindin), is a calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. Calretinin is abundantly expressed in neurons including retina (which gave it the name) and cortical interneurons. Expression was found in different neurons than that of the similar vitamin D-dependent calcium-binding protein, calbindin-28kDa. Calretinin has an important role as a modulator of neuronal excitability including the induction of long-term potentiation. Loss of expression of calretinin in hippocampal interneurons has been suggested to be relevant in temporal lobe epilepsy. It is expressed in a number of other locations including hair follicles.
背景・参考文献
1. Francavilla C et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017).
2. McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016).
サブセルラー局在
Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.
UNIPROT #
別名
29 kDa calbindin antibody
CAB 29 antibody
CAB29 antibody
CAL 2 antibody
CAL2 antibody
CALB 2 antibody
CALB2 antibody
CALB2_HUMAN antibody
Calbindin 2 29kDa antibody
Calbindin 2 antibody
詳細を見る画像
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mIHC analysis of mouse brain tissue (Frozen section) with Calretinin (HA601369, 1/1,000) and PV (ET1703-15, 1/50,000).
The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. -
mIHC analysis of mouse retina tissue (Frozen section) with Calretinin (HA601369, 1/1,000).
The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. -
Western blot analysis of Calretinin on different lysates with Mouse anti-Calretinin antibody (HA601369) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse colon tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat colon tissue lysate (20 µg/Lane)
Predicted band size: 32 kDa
Observed band size: 29 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601369) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Application: IHC-Fr
Species: Mouse
Site: Retina
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Retina
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601369, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Retina
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601369, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-tissue
Species: Mouse
Site: Hippocampus
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Calretinin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA601369 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601369 at 1/2,000 dilution. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA601369 IP in mouse brain tissue lysate
Lane 3: Mouse IgG instead of HA601369 in mouse brain tissue lysate
Blocking/Dilution buffer: Primary Antibody Dilution
Exposure time: 40 seconds; ECL: K1801
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）