Dynamin 1 Recombinant Mouse Monoclonal Antibody [A1B1-R]
概要
製品名
Dynamin 1 Recombinant Mouse Monoclonal Antibody [A1B1-R]
抗体の種類
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within human Dynamin 1 aa 500-800.
種属反応性
Mouse, Rat
検証された応用例
WB
分子量
Predicted band size: 97 kDa
陽性対照
Rat brain tissue lysate, mouse brain tissue lysate.
結合
unconjugated
クローン番号
A1B1-R
RRID
製品の特徴
形態
Liquid
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG1
精製方法
Protein A affinity purified.
応用希釈度
-
WB
-
1:1,000
ターゲット
機能
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
背景文献
1. von Spiczak S. et. al. DNM1 encephalopathy: A new disease of vesicle fission. Neurology. 2017 Jul 25;89(4):385-394.
2. Lee MW. et. al. Molecular Motor Dnm1 Synergistically Induces Membrane Curvature To Facilitate Mitochondrial Fission. ACS Cent Sci. 2017 Nov 22;3(11):1156-1167.
亜細胞局在
Cytoskeleton, cytoplasm.
別名
B dynamin antibody
D100 antibody
DNM 1 antibody
DNM antibody
DNM1 antibody
DYN1_HUMAN antibody
Dynamin antibody
Dynamin-1 antibody
Dynamin1 antibody
画像
-
Western blot analysis of Dynamin 1 on different lysates with Mouse anti-Dynamin 1 antibody (HA601230) at 1/1,000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601230) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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