iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system)
Catalog# HA1150
iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system)
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WB
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Rabbit
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iFluor™ 680se
概要
製品名
iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system)
抗体の種類
Goat Polyclonal Antibody
免疫原
Rabbit IgG (H+L).
種属反応性
Rabbit
検証された応用例
WB
結合
iFluor™ 680se
Reactivity Data
| WB | |
|---|---|
| Rabbit | |
| Species Independent |
|
製品の特徴
形態
Liquid
濃度
保存方法
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
保存用バッファー
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Immunogen affinity purified.
応用希釈度
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WB
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1:5,000-1:10,000
ターゲット
機能
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
背景文献
暂无
画像
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Western blot analysis of GAPDH and HSP90 on different lysates at 1/20,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10μM MG-132 for 6 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 25μM MG-132 for 4 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37/90 kDa
Observed band size: 37/90 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor™ 680se Secondary Antibody (HA1150) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"