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iFluor™ 647 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody

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Catalog# HA1142

iFluor™ 647 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody

Application

  • IHC-Fr

  • IF-Tissue

  • IF-Cell

Reactivity

  • Chicken

Conjugation

  • iFluor™ 647

概要

製品名

iFluor™ 647 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody

抗体の種類

Goat Polyclonal Antibody

免疫原

Chicken IgY protein.

種属反応性

Chicken

検証された応用例

IHC-Fr, IF-Tissue, IF-Cell

結合

iFluor™ 647

製品の特徴

形態

Liquid

保存方法

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

保存用バッファー

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

アイソタイプ

IgG

精製方法

Immunogen affinity purified.

応用希釈度

  • IHC-Fr

  • 1:1,000

  • IF-Tissue

  • 1:1,000

  • IF-Cell

  • 1:250-1:500

ターゲット

機能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

背景文献

暂无

画像

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Primary antibody (<a href="/products/HA601471" style="font-weight: bold;text-decoration: underline;">HA601471</a>) concentration: 1/200<br /><br />Secondary Antibody (<a href="/products/HA1142" style="font-weight: bold;text-decoration: underline;">HA1142</a>) concentration: 1/1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: cerebellum

    Sample: Frozen section

    Primary antibody (HA601471) concentration: 1/200

    Secondary Antibody (HA1142) concentration: 1/1,000

    Antigen retrieval: Not required

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Primary antibody (<a href="/products/HA601471" style="font-weight: bold;text-decoration: underline;">HA601471</a>) concentration: 1/200<br /><br />Secondary Antibody (<a href="/products/HA1142" style="font-weight: bold;text-decoration: underline;">HA1142</a>) concentration: 1/1,000

    Application: IF-Tissue

    Species: Mouse

    Site: cerebellum

    Sample: Paraffin-embedded section

    Primary antibody (HA601471) concentration: 1/200

    Secondary Antibody (HA1142) concentration: 1/1,000

  • Immunocytochemistry analysis of C6 cells labeling Iba1 with Chicken anti-Iba1 antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Chicken anti-Iba1 antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Chicken IgY (iFluor&trade; 647, <a href="/products/HA1142" style="font-weight: bold;text-decoration: underline;">HA1142</a>) was used as the secondary antibody at 1/250 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of C6 cells labeling Iba1 with Chicken anti-Iba1 antibody (HA601376) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Chicken anti-Iba1 antibody (HA601376) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Chicken IgY (iFluor™ 647, HA1142) was used as the secondary antibody at 1/250 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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