GFAP Rabbit Polyclonal Antibody

Western blot analysis of GFAP on mouse brain tissue lysates with Rabbit anti-GFAP antibody (R1308-9) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Immunofluorescent staining of A172 cells using anti- GFAP rabbit polyclonal antibody.

Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- GFAP rabbit polyclonal antibody.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GFAP antibody (R1308-9) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (R1308-9) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.