Skip to content

GST Mouse Monoclonal Antibody [16-21]

製品仕様

問い合わせをクリック

お問い合わせ

メール: [email protected]

電話: 0571-88062880

代理店を調べる

Catalog# EM80701

GST Mouse Monoclonal Antibody [16-21]

Application

  • WB

  • ELISA

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

GST Mouse Monoclonal Antibody [16-21]

抗体の種類

Mouse Monoclonal Antibody

免疫原

Recombinant protein within GST protein.

種属反応性

Species independent

検証された応用例

WB, ELISA

陽性対照

GST-tagged recombinant protein.

結合

unconjugated

クローン番号

16-21

RRID

AB_2940896

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG1

精製方法

Protein A affinity purified.

応用希釈度

  • WB

  • 1:5,000

  • ELISA

  • 1:10,000-1:20,000

ターゲット

機能

The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.

背景文献

1. Choi, H.K., et al. 2013. Early estrogen-induced gene 1, a novel RANK signaling component, is essential for osteoclastogenesis. Cell Res. 23: 524-536.

2. Patra, A.K., et al. 2013. An alternative NFAT-activation pathway mediated by IL-7 is critical for early thymocyte development. Nat. Immunol. 14: 127-135.

別名

Glutathione S Transferase antibody

Glutathione S-transferase class-mu 26 kDa isozyme antibody

GST antibody

Sj26 antigen antibody

SjGST antibody

画像

  • Western blot analysis of GST on different lysates with Mouse anti-GST antibody (<a href="/products/EM80701" style="font-weight: bold;text-decoration: underline;">EM80701</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 46 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM80701" style="font-weight: bold;text-decoration: underline;">EM80701</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GST on different lysates with Mouse anti-GST antibody (EM80701) at 1/5,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 46 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM80701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单