ALDH2 Recombinant Mouse Monoclonal Antibody [7-D8-R]
Catalog# HA601277
ALDH2 Recombinant Mouse Monoclonal Antibody [7-D8-R]
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WB
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IF-Cell
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IHC-P
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Human
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Rat
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HA610152
不含抗保成分
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unconjugated
概要
製品名
ALDH2 Recombinant Mouse Monoclonal Antibody [7-D8-R]
抗体の種類
Recombinant Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human ALDH2 aa 1-50 / 517.
種属反応性
Human, Rat
検証された応用例
WB, IF-Cell, IHC-P
分子量
Predicted band size: 56 kDa
陽性対照
293T cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, THP-1 cell lysate, human liver tissue lysate, rat liver tissue lysate, rat testis tissue lysate, HepG2, human liver tissue, rat liver tissue.
結合
unconjugated
クローン番号
7-D8-R
RRID
製品の特徴
形態
Liquid
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG1
精製方法
Protein A affinity purified.
応用希釈度
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:2,000
ターゲット
機能
Aldehyde dehydrogenase 2 family (mitochondrial), also known as ALDH2, is a human gene found on chromosome 12. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. The ALDH2 gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Most Europeans have two major isozymes, while approximately 50% of Northeast Asians have one normal copy of the ALDH2 gene and one mutant copy that encodes an inactive mitochondrial isoenzyme. A remarkably higher frequency of acute alcohol intoxication among Northeast Asians than among Europeans has been repeatedly shown to be related to the very much reduced activity of the mutant ALDH2-2 isoenzyme.
背景文献
1. Hempel J, Kaiser R, J rnvall H (1985). "Mitochondrial aldehyde dehydrogenase from human liver. Primary structure, differences in relation to the cytosolic enzyme, and functional correlations." Eur. J. Biochem. 153 (1): 13-28. 2.Chao YC, Liou SR, Tsai SF, Yin SJ (1994). "Dominance of the mutant ALDH2(2) allele in the expression of human stomach aldehyde dehydrogenase-2 activity.". Proc. Natl. Sci. Counc. Repub. China B 17 (3): 98-102.
2. Seitz HK, Meier P (2007). "The role of acetaldehyde in upper digestive tract cancer in alcoholics.". Translational research : the journal of laboratory and clinical medicine 149 (6): 293-7.
亜細胞局在
Mitochondrion matrix.
UNIPROT #
別名
Acetaldehyde dehydrogenase 2 antibody
Aldehyde dehydrogenase 2 family (mitochondrial) antibody
Aldehyde dehydrogenase 2 family antibody
Aldehyde dehydrogenase mitochondrial antibody
Aldehyde dehydrogenase, mitochondrial antibody
ALDH 2 antibody
ALDH class 2 antibody
ALDH E2 antibody
ALDH-E2 antibody
Aldh2 antibody
展開画像
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Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (HA601277) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Lane 7: Rat liver tissue lysate (40 µg/Lane)
Lane 8: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 54 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601277) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (HA601277) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ALDH2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 54 kDa
Exposure time: 52 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601277) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling ALDH2 with Mouse anti-ALDH2 antibody (HA601277) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (HA601277) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ALDH2 antibody (HA601277) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601277) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ALDH2 antibody (HA601277) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601277) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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