PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610458
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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HA601677
含抗保成分
-
unconjugated
製品概要
製品名
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
抗体のタイプ
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within Human PARK7 aa 11-60 / 189.
交差反応性（対応種属）
Human, Mouse, Rat
検証済みアプリケーション
WB, IHC-P
標的分子量
Predicted band size: 20 kDa
ポジティブコントロール
SH-SY5Y (Human neuroblastoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
コンジュゲーション
unconjugated
クローン番号
SN07-21
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
PBS (pH7.4).
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies. Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.
背景・参考文献
1. Marín-Buera L et al. Differential proteomic profiling unveils new molecular mechanisms associated with mitochondrial complex III deficiency. J Proteomics 113:38-56 (2015).
2. Bifsha P et al. Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet 10:e1004863 (2014).
サブセルラー局在
Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus.
別名
CAP1 antibody
DJ-1 antibody
DJ1 antibody
DJ1 protein antibody
Epididymis secretory sperm binding protein Li 67p antibody
FLJ27376 antibody
FLJ34360 antibody
FLJ92274 antibody
HEL S 67p antibody
Oncogene DJ1 antibody
詳細を見る画像
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This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Western blot analysis of PARK7/DJ1 on different lysates with Rat anti-PARK7/DJ1 antibody (HA601677) at 1/5,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601677, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 20 kDa
Observed band size: 20 kDa -
This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature. -
This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature. -
This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
同一ターゲット＆同一シグナル伝達経路の関連製品
PARK7/DJ1 Recombinant Rabbit Monoclonal Antibody [SN07-21] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric)
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
