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Ki67 Rabbit Polyclonal Antibody

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Catalog# ER1706-46

Ki67 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

Ki67 Rabbit Polyclonal Antibody

抗体の種類

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within human Ki67 aa 1009-1094.

種属反応性

Human

検証された応用例

WB, IF-Cell, IHC-P, FC

分子量

Predicted band size: 359 kDa

陽性対照

HepG2, A431, A549, LOVO, human tonsil tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue.

結合

unconjugated

RRID

AB_3069061

製品の特徴

形態

Liquid

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Immunogen affinity purified.

応用希釈度

  • WB

  • 1:500-1,000

  • IF-Cell

  • 1:200-1:1,000

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:200

ターゲット

機能

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization.

背景文献

1. Cuylen S et al. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. Nature 535:308-312 (2016).

2. Booth DG et al. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife 3:E01641-E01641 (2014).

翻訳後修飾

Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.

亜細胞局在

Nucleus.

UNIPROT #

SwissProt: P46013 Human

別名

Antigen identified by monoclonal antibody Ki 67 antibody

Antigen identified by monoclonal antibody Ki-67 antibody

Antigen KI-67 antibody

Antigen KI67 antibody

Antigen Ki67 antibody

KI67_HUMAN antibody

KIA antibody

Marker of proliferation Ki-67 antibody

MIB 1 antibody

MIB antibody

展開

画像

  • ICC staining Ki67 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Ki67 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Ki67 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Ki67 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Ki67 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Ki67 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.

  • Flow cytometric analysis of Hela cells with Ki67 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Flow cytometric analysis of Hela cells with Ki67 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Western blot analysis of Ki67 on HepG2 cell lysate using anti-Ki67 antibody at 1/1,000 dilution.

    Western blot analysis of Ki67 on HepG2 cell lysate using anti-Ki67 antibody at 1/1,000 dilution.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

Filter:
  1. IHC-P
  2. IF
  3. WB

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