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HSP90 Beta Mouse Monoclonal Antibody [2-1-G3]

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Catalog# EM21103

HSP90 Beta Mouse Monoclonal Antibody [2-1-G3]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

HSP90 Beta Mouse Monoclonal Antibody [2-1-G3]

抗体のタイプ

Mouse Monoclonal Antibody

免疫原

Synthetic peptide within Human HSP90 Beta aa 311-360 / 724.

交差反応性（対応種属）

Human, Mouse, Rat

検証済みアプリケーション

WB, IHC-P

標的分子量

Predicted band size: 83 kDa

ポジティブコントロール

HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, human brain tissue, rat brain tissue, human colon carcinoma tissue, human tonsil tissue, mouse testis tissue, mouse brain tissue.

コンジュゲーション

unconjugated

クローン番号

2-1-G3

RRID

AB_3068990

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG2a

精製方法

Protein A affinity purified.

推奨希釈倍率

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:500-1:10,000

ターゲット

機能

Heat shock protein HSP 90-beta also called HSP90beta is a protein that in humans is encoded by the HSP90AB1 gene. HSP90AB1 is a molecular chaperone. Chaperones are proteins that bind to other proteins, thereby stabilizing them in an ATP-dependent manner. Chaperones stabilize new proteins during translation, mature proteins which are partially unstable but also proteins that have become partially denatured due to various kinds of cellular stress. In case proper folding or refolding is impossible, HSPs mediate protein degradation. They also have specialized functions, such as intracellular transport into organelles.

背景・参考文献

1. "Hsp90 is regulated by a switch point in the C-terminal domain."Retzlaff M., Stahl M., Eberl H.C., Lagleder S., Beck J., Kessler H., Buchner J.EMBO Rep. 10:1147-1153(2009).

2. "GCUNC-45 is a novel regulator for the progesterone receptor/hsp90 chaperoning pathway."Chadli A., Graham J.D., Abel M.G., Jackson T.A., Gordon D.F., Wood W.M., Mol. Cell. Biol. 26:1722-1730(2006)

配列相同性

Belongs to the heat shock protein 90 family.

翻訳後修飾（PTM）

Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).; ISGylated.; S-nitrosylated; negatively regulates the ATPase activity.; Phosphorylation at Tyr-301 by SRC is induced by lipopolysaccharide. Phosphorylation at Ser-226 and Ser-255 inhibits AHR interaction.; Methylated by SMYD2; facilitates dimerization and chaperone complex formation; promotes cancer cell proliferation.; Cleaved following oxidative stress resulting in HSP90AB1 protein radicals formation; disrupts the chaperoning function and the degradation of its client proteins.

サブセルラー局在

Cytoplasm

UNIPROT #

SwissProt: P08238 Human

SwissProt: P11499 Mouse

SwissProt: P34058 Rat

別名

90 kda heat shock protein beta HSP90 beta antibody

D6S182 antibody

FLJ26984 antibody

Heat shock 84 kDa antibody

Heat shock 90kD protein 1, beta antibody

Heat shock 90kDa protein 1 beta antibody

Heat shock protein 90 alpha family class B member 1 antibody

Heat shock protein 90 kDa antibody

Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody

Heat shock protein 90kDa alpha family class B member 1 antibody

詳細を見る

画像

  • Western blot analysis of HSP90 Beta on different lysates with Mouse anti-HSP90 Beta antibody (<a href="/products/EM21103" style="font-weight: bold;text-decoration: underline;">EM21103</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: K-562 cell lysate (20 µg/Lane)<br />Lane 5: Jurkat cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 7: C6 cell lysate (20 µg/Lane)<br />Lane 8: PC-12 cell lysate (20 µg/Lane)<br />Lane 9: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 10: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 11: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 83 kDa<br />Observed band size: 83 kDa<br /><br />Exposure time: 53 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM21103" style="font-weight: bold;text-decoration: underline;">EM21103</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HSP90 Beta on different lysates with Mouse anti-HSP90 Beta antibody (EM21103) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: K-562 cell lysate (20 µg/Lane)
    Lane 5: Jurkat cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: C6 cell lysate (20 µg/Lane)
    Lane 8: PC-12 cell lysate (20 µg/Lane)
    Lane 9: Mouse brain tissue lysate (40 µg/Lane)
    Lane 10: Mouse heart tissue lysate (40 µg/Lane)
    Lane 11: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

    Exposure time: 53 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21103) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 Beta antibody (<a href="/products/EM21103" style="font-weight: bold;text-decoration: underline;">EM21103</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM21103" style="font-weight: bold;text-decoration: underline;">EM21103</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 Beta antibody (EM21103) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-HSP90 Beta antibody (<a href="/products/EM21103" style="font-weight: bold;text-decoration: underline;">EM21103</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM21103" style="font-weight: bold;text-decoration: underline;">EM21103</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-HSP90 Beta antibody (EM21103) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

文献・引用論文

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