CD68 Recombinant Antibody [PSH06-65] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610399
CD68 Recombinant Antibody [PSH06-65] - Rat IgG1 (Chimeric) - BSA and Azide free
-
WB
-
IHC-Fr
-
IHC-P
-
IF-Cell
-
IF-Tissue
-
Mouse
-
Rat
-
HA601596
含抗保成分
-
unconjugated
製品概要
製品名
CD68 Recombinant Antibody [PSH06-65] - Rat IgG1 (Chimeric) - BSA and Azide free
抗体のタイプ
Recombinant Chimeric Antibody
免疫原
Recombinant protein within mouse CD68 aa 1-316.
交差反応性（対応種属）
Mouse, Rat
検証済みアプリケーション
WB, IHC-Fr, IHC-P, IF-Cell, IF-Tissue
標的分子量
Predicted band size: 35 kDa
ポジティブコントロール
RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate.
コンジュゲーション
unconjugated
クローン番号
PSH06-65
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
PBS (pH7.4).
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, Scavenger Receptor Class D, Member 1, SCARD1, and LAMP4. The mouse equivalent is known as "macrosialin".
背景・参考文献
1. Choudhary M et al. CD68: Potential Contributor to Inflammation and RPE Cell Dystrophy. Adv Exp Med Biol. 2023
2. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan
サブセルラー局在
Endosome membrane, Lysosome membrane; Cell membrane.
別名
CD 68 antibody
CD68 antibody
CD68 antigen antibody
CD68 molecule antibody
CD68_HUMAN antibody
DKFZp686M18236 antibody
gp11 antibody
Gp110 antibody
LAMP4 antibody
Macrophage antigen CD68 (microsialin) antibody
詳細を見る画像
-
☑ Cell treatment (CT)
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA601596) at 1/2,000 dilution.
Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate (10 µg/Lane)
Lane 2: RAW264.7 treated with PNGase F cell lysate (10 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (20 µg/Lane)
Exposure time: 20 seconds; ECL: K1801
CD68 is a glycoprotein and can be deglycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 7680921)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601596, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35 kDa
Observed band size: 60-100 kDa -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Spleen
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA601596, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Spleen
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA601596, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601596, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601596, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601596, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601596, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cells)/NIH/3T3 (Mouse fibroblasts)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601596, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Negative control: NIH/3T3 (PMID: 29631164). -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601596, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
This data was developed using HA601596, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601596, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）