VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
概要
製品名
VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
抗体の種類
Recombinant Chimeric Antibody
免疫原
Recombinant protein within Mouse VGLUT1 aa 1-560.
種属反応性
Mouse, Rat
検証された応用例
IHC-Fr, WB, IF-Tissue
分子量
Predicted band size: 62 kDa
陽性対照
Mouse brain tissue lysate.
結合
unconjugated
クローン番号
PSH10-53
製品の特徴
形態
Liquid
保存方法
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
保存用バッファー
PBS (pH7.4).
精製方法
Protein A affinity purified.
応用希釈度
-
IHC-Fr
-
1:500
-
WB
-
1:5,000
-
IF-Tissue
-
1:500
ターゲット
機能
Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
背景文献
1. Souter EA et al. Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration. eNeuro. 2022 Jan
2. Jin S et al. Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections. Neurochem Res. 2023 Aug
亜細胞局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome.
UNIPROT #
別名
BNPI antibody
Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
Brain specific Na dependent inorganic phosphate cotransporter antibody
Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
Slc17a7 antibody
Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
Solute carrier family 17 member 7 antibody
Vesicular glutamate transporter 1 antibody
VGLU1_HUMAN antibody
VGLUT 1 antibody
展開画像
-
-
-
Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody (HA610283) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 2: Mouse lung tissue lysate (no heat) (negative) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610283) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"