VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Catalog# HA610283
VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
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IHC-Fr
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WB
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IF-Tissue
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Mouse
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Rat
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HA601449
含抗保成分
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unconjugated
製品概要
製品名
VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
抗体のタイプ
Recombinant Chimeric Antibody
免疫原
Recombinant protein within Mouse VGLUT1 aa 1-560.
交差反応性（対応種属）
Mouse, Rat
検証済みアプリケーション
IHC-Fr, WB, IF-Tissue
標的分子量
Predicted band size: 62 kDa
ポジティブコントロール
Mouse brain tissue lysate.
コンジュゲーション
unconjugated
クローン番号
PSH10-53
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
1*PBS (pH7.4).
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
背景・参考文献
1. Souter EA et al. Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration. eNeuro. 2022 Jan
2. Jin S et al. Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections. Neurochem Res. 2023 Aug
サブセルラー局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome.
UNIPROT #
別名
BNPI antibody
Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
Brain specific Na dependent inorganic phosphate cotransporter antibody
Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
Slc17a7 antibody
Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
Solute carrier family 17 member 7 antibody
Vesicular glutamate transporter 1 antibody
VGLU1_HUMAN antibody
VGLUT 1 antibody
詳細を見る画像
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This data was developed using HA601449, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: hippocampus
Sample: Frozen section
Antibody concentration: 1/500 (VGLUT1, HA601449, Guinea pig, red); 1/500 (VGLUT2, HA601451, Mouse, green)
Antigen retrieval: Not required -
This data was developed using HA601449, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: hippocampus
Sample: Frozen section
Antibody concentration: 1/500 (VGLUT1, HA601449, Guinea pig, red); 1/500 (VGLUT2, HA601451, Mouse, green)
Antigen retrieval: Not required -
This data was developed using HA601449, the same antibody clone in a different buffer formulation.
Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody (HA601449) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 2: Mouse lung tissue lysate (no heat) (negative) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601449) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）