Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Rat IgG1 (Chimeric)
概要
製品名
Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Rat IgG1 (Chimeric)
抗体の種類
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within Human GLUT1 aa 443-492 / 492.
種属反応性
Human, Mouse, Rat
検証された応用例
IHC-Fr, IHC-P, IF-Tissue, WB
分子量
Predicted band size: 54 kDa
陽性対照
Human liver tissue, mouse liver tissue, HeLa cell lysate, HT-29 cell lysate, PC-12 cell lysate, Mouse liver tissue lysate.
結合
unconjugated
クローン番号
SA0377
製品の特徴
形態
Liquid
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
精製方法
Protein A affinity purified.
応用希釈度
-
IHC-Fr
-
1:500
-
IHC-P
-
1:2,000-1:5,000
-
IF-Tissue
-
1:500-1:1,000
-
WB
-
1:50,000
ターゲット
機能
Glucose transporter 1 (or GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein that in humans is encoded by the SLC2A1 gene. GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells. This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. One good source of GLUT1 is erythrocyte membranes. GLUT1 accounts for 2 percent of the protein in the plasma membrane of erythrocytes. GLUT1, found in the plasma membrane of erythrocytes, is a classic example of a uniporter. After glucose is transported into the erythrocyte, it is rapidly phosphorylated, forming glucose-6-phosphate, which cannot leave the cell. Mutations in this gene can cause GLUT1 deficiency syndrome 1, GLUT1 deficiency syndrome 2, idiopathic generalized epilepsy 12, dystonia 9, and stomatin-deficient cryohydrocytosis.
背景文献
1. Boyer-Di Ponio J et al. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS One 9:e84179 (2014).
2. Saucillo DC et al. Leptin metabolically licenses T cells for activation to link nutrition and immunity. J Immunol 192:136-44 (2014).
亜細胞局在
Cell membrane, Melanosome
別名
Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
CSE antibody
DYT17 antibody
DYT18 antibody
DYT9 antibody
EIG12 antibody
erythrocyte/brain antibody
Erythrocyte/hepatoma glucose transporter antibody
facilitated glucose transporter member 1 antibody
Glucose transporter 1 antibody
展開画像
-
Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601521) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601521) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of Glucose Transporter GLUT1 on different lysates with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/50,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HT-29 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Mouse liver tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 45-60 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601521) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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