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Beta III Tubulin Recombinant Antibody [PSH15-32] - Rat IgG1 (Chimeric) - BSA and Azide free

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Catalog# HA610445

Beta III Tubulin Recombinant Antibody [PSH15-32] - Rat IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-P

  • IF-Tissue

  • IHC-Fr

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

製品概要

製品名

Beta III Tubulin Recombinant Antibody [PSH15-32] - Rat IgG1 (Chimeric) - BSA and Azide free

抗体のタイプ

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within mouse Beta III Tubulin aa 401-450.

交差反応性（対応種属）

Human, Mouse, Rat

検証済みアプリケーション

IHC-P, IF-Tissue, IHC-Fr, WB, IF-Cell

標的分子量

Predicted band size: 50 kDa

ポジティブコントロール

Neuro-2a, Human Cerebellum, Mouse Cerebellum, Rat Cerebellum.

コンジュゲーション

unconjugated

クローン番号

PSH15-32

製品の特徴

形態

Liquid

濃度

保存方法

Store at 2-8℃. Avoid freeze.

保存バッファー

PBS (pH7.4).

精製方法

Protein A affinity purified.

推奨希釈倍率

ターゲット

機能

Tubulin beta-3 chain, Class III β-tubulin, βIII-tubulin (β3-tubulin) or β-tubulin III, is a microtubule element of the tubulin family found almost exclusively in neurons, and in testis cells. In humans, it is encoded by the TUBB3 gene. Forebrain neuronal culture after 40 days of differentiation from induced human pluripotent stem cells. Class III β-tubulin is one of the seven β-tubulin isotypes identified in the human genome, predominantly in neurons and the testis. It is conditionally expressed in a number of other tissues after exposure to a toxic microenvironment featured by hypoxia and poor nutrient supply. Posttranslational changes including phosphorylation and glycosylation are required for functional activity. Class III β-tubulin's role in neural development has warranted its use as an early biomarker of neural cell differentiation from multi potent progenitors. TUBB3 inactivation impairs neural progenitor proliferation. Rescue experiments demonstrate the non-interchangeability of TUBB3 with other classes of β-tubulins which cannot restore the phenotype resulting from TUBB3 inactivation. Congenital neurologic syndromes associated with TUBB3 missense mutations demonstrate the critical importance of class III β-tubulin for normal neural development.

背景・参考文献

1. Puri D et al. TUBB3 and KIF21A in neurodevelopment and disease. Front Neurosci. 2023 Aug

2. Jin S et al. TUBB3 M323V Syndrome Presents with Infantile Nystagmus. Genes (Basel). 2021 Apr

サブセルラー局在

Cytoplasm, cytoskeleton, growth cone, lamellipodium, filopodium.

UNIPROT #

SwissProt: Q13509 Human

SwissProt: Q9ERD7 Mouse

SwissProt: Q4QRB4 Rat

別名

beta 3 tubulin antibody

beta 4 antibody

beta-4 antibody

CDCBM antibody

CDCBM1 antibody

CFEOM3 antibody

CFEOM3A antibody

FEOM3 antibody

M(beta)3 antibody

M(beta)6 antibody

詳細を見る

画像

  • This data was developed using <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunocytochemistry (IF-cell)<br /><br />Species: Mouse<br />Sample: Neuro-2a (Mouse brain neuroblastoma cell)<br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br /><br />Primary antibody: <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, 1/50, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 45 minutes at room temperature.<br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    This data was developed using HA601655, the same antibody clone in a different buffer formulation.
    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: Neuro-2a (Mouse brain neuroblastoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA601655, 1/50, overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
    Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • This data was developed using <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    This data was developed using HA601655, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601655, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • This data was developed using <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    This data was developed using HA601655, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601655, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • This data was developed using <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    This data was developed using HA601655, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601655, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • This data was developed using <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Human <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, 1/1,000, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 1.5 hours at room temperature.

    This data was developed using HA601655, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Human
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA601655, 1/1,000, overnight at 4°C.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.

  • This data was developed using <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, 1/1,000, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 1.5 hours at room temperature.

    This data was developed using HA601655, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA601655, 1/1,000, overnight at 4°C.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.

  • This data was developed using <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Rat <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601655" style="font-weight: bold;text-decoration: underline;">HA601655</a>, 1/1,000, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 1.5 hours at room temperature.

    This data was developed using HA601655, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA601655, 1/1,000, overnight at 4°C.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

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