p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72] - BSA and Azide free
Catalog# HA750906
p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72] - BSA and Azide free
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WB
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Human
-
Mouse
-
Rat
-
Monkey
概要
製品名
p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72] - BSA and Azide free
抗体の種類
Recombinant Rabbit multiclonal
免疫原
Recombinant protein within human MAPK12 aa 1-367 / 367.
種属反応性
Human, Mouse, Rat, Monkey
検証された応用例
WB
分子量
Predicted band size: 42 kDa
陽性対照
293T cell lysate, HeLa cell lysate, K-562 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate.
結合
unconjugated
クローン番号
PSH03-72
製品の特徴
形態
Liquid
保存方法
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
保存用バッファー
PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
応用希釈度
-
WB
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1:1,000
ターゲット
機能
A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs). p38 MAP Kinase Antibody detects endogenous levels of total p38 alpha, p38 beta, p38 gamma, p38 delta protein.
背景文献
1. Wang J et al. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. Oncol Lett. 2022 Oct
2. Yang R et al. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. Int J Mol Sci. 2023 Jun
亜細胞局在
Cytoplasm, Nucleus, Mitochondrion.
UNIPROT #
別名
ERK 6 antibody
ERK-6 antibody
ERK6 antibody
Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody
MAPK 12 antibody
Mapk12 antibody
Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase p38 gamma antibody
展開画像
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Western blot analysis of p38 MAPK on different lysates with Rabbit anti-p38 MAPK antibody (HA750906) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 4 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750906) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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