Hsp27 Mouse Monoclonal Antibody [10-8]

機能

Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, α-crystallin,Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and?cell differentiation. They also take part in signal transduction. The main function of Hsp27 is to provide thermotolerance in vivo, cytoprotection, and support of cell survival under stress conditions. More specialized functions of Hsp27 are manifold and complex. In vitro it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding by the Hsp70-complex. Hsp27 is also involved in the apoptotic signalling pathway. Hsp27 interacts with the outer mitochondrial membranes and interferes with the activation of?cytochrome c/Apaf-1/dATP complex and therefore inhibits the activation of procaspase-9.

背景文献

1. Heat shock protein 27 is associated with freedom from graft vasculopathy after human cardiac transplantation." De Souza A.I.,Wait R.,Mitchell A.G.,Banner N.R.,Dunn M.J.,Rose M.L. Circ. Res. 97:192-198(2005)

2. PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5." Kostenko S.,Johannessen M.,Moens U. Cell. Signal. 21:712-718(2009)

3. The mutational spectrum in a cohort of Charcot-Marie-Tooth disease type 2 among the Han Chinese in Taiwan." Lin K.P., Soong B.W.,Yang C.C.,Huang L.W.,Chang M.H., Lee I.H.,Antonellis A.,Lee Y.C. PLoS ONE 6:E29393-E29393(2011)

配列相同性

Belongs to the small heat shock protein (HSP20) family.

組織特異性

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

翻訳後修飾

Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.

亜細胞局在

Cytoplasm, nucleus

UNIPROT #

SwissProt: P04792 Human

別名